ZFIN ID: ZDB-FIG-140107-44
Reichert et al., 2013 - A BMP regulatory network controls ectodermal cell fate decisions at the neural plate border. Development (Cambridge, England)   140(21):4435-4444 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Knockdown Reagents:
Anatomical Terms:
Stage Range: 75%-epiboly to 5-9 somites

Fig. 1 Distinct domains of BMP activity exist at the NPB. (A) (Left) In situ hybridisation (ISH) for mRFP and sox10 in Tg(BRE:mRFP) zebrafish embryos. The 80% epiboly image is a lateral view, ventral to the left. Somitogenesis stages are shown in dorsal anterior view. Black arrow, outer domain of BMP activity; red arrow, inner NC domain. (Right) Double-fluorescent in situ hybridisation (DFISH) using probes against mRFP (Fast Blue, red) and sox10 (fluorescein-TSA, green). Arrows indicate the two distinct domains of BMP activity. Dorsal view, with anterior top right. (B) (Left) sox10 and krox20 ISH on Tg(hsp70l:dnXla.Bmpr1a-GFP) embryos heat shocked at the indicated stages. Brackets indicate the width of the neural ectoderm marked by krox20. (Right) Western blots of 5-somite stage Tg(hsp70l:dnXla.Bmpr1a-GFP) embryos heat shocked (hs) at bud stage, using the antibodies indicated. The control (cont.) corresponds to sibling embryos that were not heat shocked. (C) ISH for gdf6a and bmp6 at the stages indicated. (D) DFISH for sox10 and gdf6a. Dorsal view, with anterior to top. (E,F) Wild-type or Tg(BRE:mRFP) embryos were injected with 10 ng control MO or MOs against gdf6a (E) or bmp6 (F) and stained for sox10 or mRFP. Red arrows indicate the inner domain of BMP signalling as visualised by mRFP. The number of embryos with the observed phenotype among total embryos examined for a representative experiment is given bottom right. V, ventral; D, dorsal; A, anterior; P, posterior.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
bmp6 WT standard conditions Bud Not Detected whole organism ISH
1-4 somites Not Detected whole organism ISH
5-9 somites ectoderm ISH
egr2b w30Tg standard conditions 5-9 somites presumptive rhombomere 3 ISH
5-9 somites presumptive rhombomere 5 ISH
w30Tg heat shock 5-9 somites presumptive rhombomere 3 ISH
5-9 somites presumptive rhombomere 5 ISH
gdf6a WT standard conditions 1-4 somites ectoderm anterior region ISH
1-4 somites neural crest ISH
Bud Not Detected whole organism ISH
5-9 somites ectoderm anterior region ISH
5-9 somites neural crest ISH
mRFP cj100Tg control 5-9 somites ectoderm ISH
5-9 somites neural crest ISH
cj100Tg standard conditions 75%-epiboly to 90%-epiboly whole organism ventral side ISH
1-4 somites ectoderm ISH
1-4 somites neural crest ISH
5-9 somites ectoderm ISH
5-9 somites neural crest ISH
cj100Tg + MO1-bmp6 standard conditions 5-9 somites ectoderm ISH
5-9 somites neural crest ISH
cj100Tg + MO1-gdf6a standard conditions 5-9 somites ectoderm ISH
5-9 somites neural crest ISH
cj100Tg + MO5-gdf6a standard conditions 5-9 somites ectoderm ISH
5-9 somites neural crest ISH
sox10 WT control 5-9 somites neural crest ISH
WT + MO1-bmp6 standard conditions 5-9 somites neural crest ISH
WT + MO1-gdf6a standard conditions 5-9 somites neural crest ISH
WT + MO5-gdf6a standard conditions 5-9 somites neural crest ISH
cj100Tg standard conditions 1-4 somites neural crest ISH
5-9 somites neural crest ISH
w30Tg standard conditions 5-9 somites neural crest ISH
w30Tg heat shock 5-9 somites neural crest ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development