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Embryonic Expression of ppp1cba and ppp1cbb during zebrafish development. Detection of ppp1cba and ppp1cbb mRNA was carried out by whole-mount in situ hybridization using gene-specific probes on staged embryos from 256 cells to 24 hpf. Images are lateral views, animal pole at top. (A–L) ppp1cb transcripts are ubiquitously expressed at early developmental stages, 256 cells stage (A and F), sphere stage (B and G), shield stage (C and H), bud stage (D and I) and 24 hpf (K and L). A probe for ppp1cba was used in (A–D and K) while ppp1cbb was used for (F–I and L). Negative control sense probes for ppp1cba and ppp1cbb did not show staining (E and J). Gene specific primers were used to detect ppp1cba and ppp1cbb in various stages of development by RT-PCR. (M) Both genes were expressed maternally and zygotically throughout early development. Amplification of eF1&alpha and total RNA without addition of reverse transcriptase were used as controls.
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