ZFIN ID: ZDB-FIG-131213-37
Lam et al., 2013 - odd-skipped related 2 is required for fin chondrogenesis in zebrafish. Developmental dynamics : an official publication of the American Association of Anatomists   242(11):1284-92 Full text @ Dev. Dyn.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage Range: Prim-5 to Long-pec
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage Range: Prim-5 to Long-pec

Fig. S4 Pronephric kidney patterning and structure is normal in osr2 morphants. A–N: In situ hybridization of wild-type (A, C, E, G, I) and osr2 morphant embryos (B, D, F, H, J) using pronephric nephron markers. pax2a (A–F) is expressed in the entire pronephric nephron, ae2 (G and H) and nbc1 (I and J) are expressed in the proximal nephron, trpm7 (K, L) are expressed in the early distal segment and ret1 (M, N) is expressed in the distal segment of the nephron. Embryonic stages are indicated on the corresponding panels. O, P: a6F immunohistochemistry on wild-type (O) and osr2 morphant (P) showing normal expression of Na+/K+-ATPase in the pronephros.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
pax2a WT control Prim-15 pronephric duct ISH
Long-pec pronephric duct ISH
WT + MO3-osr2 + MO4-osr2 standard conditions Prim-15 pronephric duct ISH
Long-pec pronephric duct ISH
ret WT control Prim-5 posterior pronephric duct ISH
WT + MO3-osr2 + MO4-osr2 standard conditions Prim-5 posterior pronephric duct ISH
slc4a2a WT control Prim-5 pronephric proximal convoluted tubule ISH
Prim-5 pronephric proximal straight tubule ISH
WT + MO3-osr2 + MO4-osr2 standard conditions Prim-5 pronephric proximal convoluted tubule ISH
Prim-5 pronephric proximal straight tubule ISH
slc4a4a WT control Prim-5 pronephric proximal convoluted tubule ISH
Prim-5 pronephric proximal straight tubule ISH
WT + MO3-osr2 + MO4-osr2 standard conditions Prim-5 pronephric proximal convoluted tubule ISH
Prim-5 pronephric proximal straight tubule ISH
trpm7 WT control Prim-5 pronephric proximal straight tubule ISH
WT + MO3-osr2 + MO4-osr2 standard conditions Prim-5 pronephric proximal straight tubule ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO3-osr2 + MO4-osr2 standard conditions Prim-5 intestinal rod morphology, normal
Prim-5 pronephric duct morphology, normal
Prim-15 intestinal rod morphology, normal
Prim-15 pronephric duct morphology, normal
Long-pec intestinal rod morphology, normal
Long-pec pronephric duct morphology, normal
Acknowledgments:
ZFIN wishes to thank the journal Developmental dynamics : an official publication of the American Association of Anatomists for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Dev. Dyn.