FIGURE

Fig. S4

ID

ZDB-FIG-131213-37

Publication

Lam et al., 2013 - odd-skipped related 2 is required for fin chondrogenesis in zebrafish

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Fig. S4

Pronephric kidney patterning and structure is normal in osr2 morphants. A–N: In situ hybridization of wild-type (A, C, E, G, I) and osr2 morphant embryos (B, D, F, H, J) using pronephric nephron markers. pax2a (A–F) is expressed in the entire pronephric nephron, ae2 (G and H) and nbc1 (I and J) are expressed in the proximal nephron, trpm7 (K, L) are expressed in the early distal segment and ret1 (M, N) is expressed in the distal segment of the nephron. Embryonic stages are indicated on the corresponding panels. O, P: a6F immunohistochemistry on wild-type (O) and osr2 morphant (P) showing normal expression of Na⁺/K⁺-ATPase in the pronephros.

Expression Data

Genes:	pax2a ret slc4a2a slc4a4a trpm7
Fish:	WT WT + MO3-osr2 + MO4-osr2
Knockdown Reagents:	MO3-osr2 MO4-osr2
Anatomical Terms:	posterior pronephric duct pronephric duct pronephric proximal convoluted tubule pronephric proximal straight tubule
Stage Range:	Prim-5 to Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO3-osr2 + MO4-osr2
Knockdown Reagents:	MO3-osr2 MO4-osr2
Observed In:	intestinal rod pronephric duct
Stage Range:	Prim-5 to Long-pec

Phenotype Detail

Acknowledgments

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