FIGURE

Fig. 8

ID

ZDB-FIG-131009-28

Publication

Drummond et al., 2013 - The role of Zic transcription factors in regulating hindbrain retinoic acid signaling

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Fig. 8

Zic2a and Zic2b depletion reduces RA signaling levels. Tg(12xRARE-ef1a:eGFP)^sk71 transgenic embryos were used to assay the level of RA signaling. To increase sensitivity of transgene detection, in situ hybridization for eGFP was employed. Control p53MO-injected embryos (A, B) were compared with Zic2a;2b;p53MO-injected embryos (C, D). Quantification of reduction in eGFP in Zic2a;2b;p53 morphants is displayed graphically with reduced expression in 75 ± 2% of embryos (p-value = 0.0004; unpaired t-test). Cyp26a1 (F) and Aldh1a2 (G) morpholinos were also injected into Tg(12xRARE-ef1a:eGFP)^sk71 embryos to compare effects with those obtained using Zic morpholinos. Images are of 26 hpf embryos in lateral (A, C, F, G) and dorsal (B, D) views with anterior to left and top, respectively.

Expression Data

Gene:	EGFP
Fish:	sk71Tg sk71Tg + MO1-cyp26a1 sk71Tg + MO2-aldh1a2 sk71Tg + MO2-zic2b + MO3-zic2a + MO4-tp53
Knockdown Reagents:	MO1-cyp26a1 MO2-aldh1a2 MO2-zic2b MO3-zic2a MO4-tp53
Anatomical Terms:	hindbrain posterior region optic cup dorsal region spinal cord
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	sk71Tg + MO2-aldh1a2 sk71Tg + MO2-zic2b + MO3-zic2a + MO4-tp53
Knockdown Reagents:	MO2-aldh1a2 MO2-zic2b MO3-zic2a MO4-tp53
Observed In:	retinoic acid receptor signaling pathway
Stage:	Prim-5

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ BMC Dev. Biol.