FIGURE

Fig. 7

ID

ZDB-FIG-131009-27

Publication

Drummond et al., 2013 - The role of Zic transcription factors in regulating hindbrain retinoic acid signaling

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Fig. 7

Validation of RARE:eGFP reporter. Tg(12xRARE-ef1a:eGFP)^sk71 transgenic embryos (described in A) were evaluated for altered signals when RA signaling was manipulated. Embryos were treated with a vehicle control, DMSO (B, E, H, H′, K, N), 5 nM all-trans retinoic acid (C, F, I, I′, L, O), or 5 μM DEAB (D, G, J, J′, M, P). We note an expansion of hindbrain/spinal cord expression of the transgene in 5 nM RA-treated embryos, and a complete loss in 5 μM DEAB-treated embryos. Altered expression is detectable by examination of fluorescence using a stereomicroscope (B-G), confocal microscope (H-J′), or by in situ hybridization with a probe to eGFP(K-P). All embryos are shown in lateral view at 48 hpf (B-M) or 26 hpf (N-P). The inset panels (H′-J′) display reporter activity in the ventral retina.

Expression Data

Gene:	EGFP
Fish:	sk71Tg
Conditions:	chemical treatment: 4-(diethylamino)benzaldehyde chemical treatment: all-trans-retinoic acid
Anatomical Terms:	hindbrain hindbrain posterior region retina dorsal region retina ventral region spinal cord
Stage Range:	Prim-5 to Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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