FIGURE

Fig. S2

ID

ZDB-FIG-130830-8

Publication

Ninov et al., 2013 - Metabolic Regulation of Cellular Plasticity in the Pancreas

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Fig. S2

Differentiation of β Cells from Notch Responsive Cells during Juvenile Stages, Related to Figure 2

(A and B) Tg(TP1:CreERT2);Tg(ubi3C:loxp-GFP-loxp:mCherry) larvae were treated with EtOH as vehicle (A) or 4-OHT (B) at 14dpf for 16h, fixed at 17dpf, and stained for 2F11 to mark the IPD cells. 4-OHT treatment resulted in the mosaic labeling of IPD cells (mCherry⁺; 2F11⁺ cells).
(C) Tg(TP1:CreERT2);Tg(insulin:loxP:mCherry-STOP:loxP:H2BGFP) larvae treated with 4-OHT at 15dpf for 24h; the pancreata were analyzed at 18dpf without switching to a high-calorie diet, and after staining with anti-GFP antibodies. No H2BGFP⁺ β-cells were observed in the SIs (19 islets in 30 animals).
(D) Tg(TP1:CreERT2);Tg(insulin:loxP:mCherry-STOP:loxP:H2BGFP) larvae treated with 4-OHT at 2.5dpf for 24h; the pancreata were analyzed at 32dpf. The SIs (arrowheads) are composed of mCherry⁺ β-cells as well as H2BGFP⁺ β-cells. Note that the H2BGFP+ β-cells in the SIs formed groups of several cells, indicating that they underwent amplification after differentiation. Scale bars, 20 μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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