ZFIN ID: ZDB-FIG-130815-18
Patterson et al., 2013 - Interactions with Iridophores and the Tissue Environment Required for Patterning Melanophores and Xanthophores during Zebrafish Adult Pigment Stripe Formation. PLoS Genetics   9(5):e1003561 Full text @ PLoS Genet.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: Days 7-13 to Adult

Fig. 6

csf1a and csf1b were expressed by interstripe iridophores as well as hypodermal and fin cells.

(A) RT-PCR of isolated iridophores (irid) and skin containing pigment cells for the iridophore marker pnp4a as well as csf1a, csf1b and kitlga. –, no template control. See text for details. (B) A larva (~6 SSL) imaged to show iridophores prior to fixation (upper) and after whole-mount staining for csf1a transcript. Not all iridophore reflecting platelets are visible and platelets that are apparent may not precisely delineate cell bodies and processes. (C,D) Whole-mount larvae (~8.5 SSL) stained for csf1a transcript. (C) csf1a was expressed in the posterior trunk at the base of the caudal fin (arrow) where a patch of posterior iridophores develops [45] and also within the fin (f). (D) csf1a staining near the horizontal myoseptum (arrow). (E–J) In situ hybridizations on vibratome cross-sections through the midtrunk (~7 SSL). (E,E2) pnp4a staining indicated iridophore locations (arrowheads) within the hypodermis of wild-type (bnc2/+) larvae (E) and revealed fewer of these cells in bnc2 mutants (E2). Arrow, melanophore. (F,F2) csf1a staining (arrowheads) was reduced in bnc2 mutants. (G–H) Staining for csf1a in wild-type (ltk/+) and ltk mutants, which lack iridophores. (G,G2) csf1a staining was absent in ltk mutants at the location where iridophores are found in the wild-type (arrowhead). (H,H2) In the fins, however, iridophore-independent csf1a expression was present in both wild-type and ltk mutant larvae. (I–J) csf1b expression was at the limit of detection by in situ hybridization. (I,I2) Along the lateral trunk, csf1b transcript (arrowheads) was evident in wild-type larvae, representing either hypodermal cells, iridophores or both, but transcript was not apparent in bnc2 mutant sections stained for equivalent times. (J,J2) Along the dorsal trunk, csf1b transcripts (arrowheads) were evident in both wild-type and bnc2 mutants. Scale bars: in (B) 60 μm for (B); in (C) 100 μm for (C); in (D) 100 μm for (D); in (E) 80 μm for (E,E2,F,F2,G,G2,I,I2), in (H) 80 μm for (H,H2); in (J) 20 μm for (J,J2).

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
csf1a WT standard conditions Days 7-13 to Adult integument RTPCR
Days 7-13 to Adult iridophore RTPCR
Days 7-13 to Adult iridophore ISH
bnc2utr16e1/utr16e1 standard conditions Days 7-13 to Adult iridophore ISH
ltkj9s1/j9s1 standard conditions Days 7-13 to Adult Not Detected iridophore ISH
csf1b WT standard conditions Days 7-13 to Adult integument RTPCR
Days 7-13 to Adult iridophore RTPCR
Days 7-13 to Adult iridophore ISH
bnc2utr16e1/utr16e1 standard conditions Days 7-13 to Adult iridophore ISH
kitlga WT standard conditions Days 7-13 to Adult integument RTPCR
pnp4a WT standard conditions Days 7-13 to Adult integument RTPCR
Days 7-13 to Adult iridophore RTPCR
Days 7-13 to Adult iridophore ISH
bnc2utr16e1/utr16e1 standard conditions Days 7-13 to Adult iridophore ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS Genet.