ZFIN ID: ZDB-FIG-130814-8
Reimer et al., 2013 - Dopamine from the Brain Promotes Spinal Motor Neuron Generation during Development and Adult Regeneration. Developmental Cell   25(5):478-491 Full text @ Dev. Cell
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Knockdown Reagents:
Anatomical Terms:
Stage: Prim-15
PHENOTYPE:
Fish:
Condition:
Knockdown Reagents:
Observed In:
Stage: Prim-15

Fig. 3 The D4a Receptor Is Necessary to Mediate the Action of Dopamine on Neurogenesis (A) PCR analysis on fluorescence-activated cell-sorted cells from olig2:dsRed/HB9:GFP double-transgenic embryos shows that d4a receptor mRNA is enriched in pMN progenitors, compared to motor neurons. (B) In a dorsal view of double-transgenic embryos, olig2:dsRed+/HB9:GFP- pMN progenitors (brackets) and double-labeled motor neurons are visible. (C) d4a morpholino knockdown is efficient, as shown by PCR. (D) d4a knockdown (MO1) reduces the number of HB9+ motor neurons, which cannot be rescued by pergolide (exposure 24–33 hpf). (E) Quantification of motor neurons in (D) (ANOVA, p = 0.0003 with Bonferroni’s posttest, p < 0.01, p < 0.0001). (F) d4a knockdown increases the number of vsx1:GFP+ interneurons (p < 0.0001). Error bars represent SEM. Scale bars, 50 μm (B, D, and F). See also Figure S3 and Table S1.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
DsRed2 ml2Tg; vu19Tg standard conditions Prim-15 spinal cord neuron IFL
GFP ml2Tg control Prim-15 spinal cord motor neuron IFL
ml2Tg + MO1-drd4a chemical treatment: drug Prim-15 spinal cord motor neuron IFL
ml2Tg + MO1-drd4a standard conditions Prim-15 spinal cord motor neuron IFL
ml2Tg; vu19Tg standard conditions Prim-15 spinal cord neuron IFL
nns5Tg control Prim-15 spinal cord interneuron IFL
nns5Tg + MO1-drd4a + MO2-drd4a standard conditions Prim-15 spinal cord interneuron IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
ml2Tg + MO1-drd4a chemical treatment: drug Prim-15 spinal cord motor neuron decreased amount, abnormal
ml2Tg + MO1-drd4a standard conditions Prim-15 spinal cord motor neuron decreased amount, abnormal
nns5Tg + MO1-drd4a + MO2-drd4a standard conditions Prim-15 spinal cord interneuron increased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Cell for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Cell, 25(5), Reimer, M.M., Norris, A., Ohnmacht, J., Patani, R., Zhong, Z., Dias, T.B., Kuscha, V., Scott, A.L., Chen, Y.C., Rozov, S., Frazer, S.L., Wyatt, C., Higashijima, S., Patton, E.E., Panula, P., Chandran, S., Becker, T., and Becker, C.G., Dopamine from the Brain Promotes Spinal Motor Neuron Generation during Development and Adult Regeneration, 478-491, Copyright (2013) with permission from Elsevier. Full text @ Dev. Cell