ZFIN ID: ZDB-FIG-130814-7
Reimer et al., 2013 - Dopamine from the Brain Promotes Spinal Motor Neuron Generation during Development and Adult Regeneration. Developmental Cell   25(5):478-491 Full text @ Dev. Cell
ADDITIONAL FIGURES
EXPRESSION / LABELING:
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Anatomical Term:
Stage: Prim-15

Fig. 2 Dopamine Signaling Inversely Affects Motor Neuron and V2 Interneuron Numbers Lateral trunk views at 33 hpf are shown (drug exposure: 24–33 hpf). (A) Treatment with the dopamine agonist pergolide leads to an increase of HB9 immunoreactive motor neurons and a reduction of transgenically labeled presumptive V2 interneurons (vsx1:GFP) in the same embryos. A small subpopulation of double-labeled cells is also strongly reduced (Mann-Whitney U test; p < 0.0001, p < 0.001). (B) NPA, another dopamine agonist, increases the number of HB9:GFP+ motor neurons and decreases the number of vsx1:GFP+ cells (left column). The D4 antagonist L-745870 has the inverse effect on numbers of these cell types (right column). Error bars represent SEM. Scale bars, 50 μm. See also Figure S2.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
GFP ml2Tg control Prim-15 spinal cord neuron IFL
ml2Tg chemical treatment: drug Prim-15 spinal cord neuron IFL
ml2Tg chemical treatment: pharmaceutical Prim-15 spinal cord neuron IFL
nns5Tg control Prim-15 spinal cord neuron IFL
nns5Tg chemical treatment: drug Prim-15 spinal cord neuron IFL
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-mnx1 IHC nns5Tg control Prim-15 spinal cord neuron
IHC nns5Tg chemical treatment: drug Prim-15 spinal cord neuron
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Developmental Cell for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Cell, 25(5), Reimer, M.M., Norris, A., Ohnmacht, J., Patani, R., Zhong, Z., Dias, T.B., Kuscha, V., Scott, A.L., Chen, Y.C., Rozov, S., Frazer, S.L., Wyatt, C., Higashijima, S., Patton, E.E., Panula, P., Chandran, S., Becker, T., and Becker, C.G., Dopamine from the Brain Promotes Spinal Motor Neuron Generation during Development and Adult Regeneration, 478-491, Copyright (2013) with permission from Elsevier. Full text @ Dev. Cell