|
Fig. S4 Cyclopamine treatment rescues the miR-30 morpholino phenotype. To achieve phenotypic rescue of the miR-30 morpholino phenotype cyclopamine was used at a concentration range of 100 μM-6.25 μM. At 6.25 μM the miR-30 morpholino phenotype improved to resemble the wild type phenotype with elongation of the tail and improved somite structure (F). Cyclopamine was dissolved in DMSO and both wild type and miR-30 morpholino injected embryos were treated with DMSO as a negative control (A–D) which had no effect on embryo development when compared to untreated. Wild type embryos treated with 6.25 μM cyclopamine showed a mild phenotype associated with Hh pathway inactivation with U shaped somites and a loss of brain chamber definition (E).