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Fig. 3

ID

ZDB-FIG-130801-3

Publication

Teslaa et al., 2013 - Zebrafish Zic2a and Zic2b regulate neural crest and craniofacial development

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Fig. 3

Zic2a regulates patterning of anterior chondrogenic condensations. 2 dpf zic2a morphants (A–F) and overexpressors (G,H) stained by ISH for col2a1a or sox9a. (A,B) Sox9a expression is reduced in the posterior PAs of Zic2a-depleted embryos (18/20, 1 exp.). (C,D) Zic2a morphants have shortened trabeculae (18/20, 1 exp.) and lack the medial ethmoid plate (12/20, 1 exp., arrow in 3C). (E,F) Expression of col2a1a reiterates the shortened trabeculae and lack of ethmoid plate in Zic2a-depleted embryos (43/43, 2 exp). (G,H) Col2a1a expression in wild-type siblings shows the bilateral trabeculae and ethmoid plate (67/107, 2 exp.). (H) The ethmoid plate is ablated and trabeculae are shortened by Zic2aYFP misexpression induced at 10 hpf (38/67, 2 exp). (A,B) are lateral views. (C–H) are ventral views, anterior to the left.

Expression Data

Genes:	col2a1a sox9a
Fish:	WT WT + MO3-zic2a uw6Tg; vu22Tg
Condition:	heat shock
Knockdown Reagent:	MO3-zic2a
Anatomical Terms:	ethmoid cartilage neurocranial trabecula neurocranium pharyngeal arch pharyngeal arch 1 pharyngeal arch 2
Stage:	Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO3-zic2a uw6Tg; vu22Tg
Condition:	heat shock
Knockdown Reagent:	MO3-zic2a
Observed In:	embryonic neurocranium morphogenesis embryonic viscerocranium morphogenesis ethmoid cartilage ethmoid cartilage medial region neurocranial trabecula pharyngeal arch
Stage:	Long-pec

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 380(1), Teslaa, J.J., Keller, A.N., Nyholm, M.K., and Grinblat, Y., Zebrafish Zic2a and Zic2b regulate neural crest and craniofacial development, 73-86, Copyright (2013) with permission from Elsevier. Full text @ Dev. Biol.