Fig. S7

The oropharynx is mildly mispatterned in zic2a morphants. Embryos stained by ISH for pitx2a or apoeb at 3 dpf. (A,B) Pitx2a expression is increased in the pharyngeal epithelium following Zic2a knockdown (49/56, 4 exp.). (C,D). apoeb, another marker of the oropharyngeal epithlium, shows a similar reduction in stomodeal size in moderately affected zic2a morphants (6/23, 2 exp.), while more severely affected embryos have no apoeb expression in the oropharnx (15/23, not shown). (E,F) apoeb is mildly mispatterned in zic2aAUGMO-injected embryos, either with increased, continual expression in the ventral stomodeum (8/48, 2 exp, arrows in F) or elongation of the stomodeum (25/48, 2 expt., not shown). (G,H) After Zic2aYFP induction at 10 hpf, the stomodeal apoeb expression domain is variably mispatterned: narrowed and lengthened (14/49, 2 exp., see H), tightened into a smaller ring (7/49, 2 exp.) or fused into a single spot (25/49, 2 exp.). A,B are lateral views. C–H are ventral views, anterior to the left.