ZFIN ID: ZDB-FIG-130719-30
Provost et al., 2013 - Multiple ribosomal proteins are expressed at high levels in developing zebrafish endoderm and are required for normal exocrine pancreas development. Zebrafish   10(2):161-169 Full text @ Zebrafish
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Protruding-mouth

Fig. 4 Morpholino-mediated knockdown of p53 does fail to rescue defective expansion of ptf1a-expressing pancreas progenitor cells in rpl mutant embryos. (A) Injection of embryos produced by rpl23ahi2582/ + ;ptf1a:eGFPjh1;ins:mCherryjh2 incross with 1 ng p53ATG MO does not rescue hypoplastic pancreatic phenotype. (B) Incidence of abnormal pancreas development among embryos produced by rpl23ahi2582/ + ;ptf1a:eGFPjh1;ins:mCherry jh2 incross injected with either p53ATG MO or mock control (phenotypic, red; wild type, blue). (C) Injection of embryos produced by rpl6hi3655b/ + ;ptf1a:eGFPjh1;ins:mCherryjh2 incross with 1 ng p53ATG MO does not rescue hypoplastic pancreatic phenotype. (D) Incidence of abnormal pancreas development among embryos produced by rpl6hi3655b/ + ;ptf1a:eGFPjh1;ins:mCherry jh2 incross injected with either p53ATG MO or mock control (phenotypic, red; wild type, blue). Color images available online at www.liebertpub.com/zeb

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
rpl23ahi2582Tg/+; jh1Tg; jh2Tg + MO4-tp53 standard conditions Protruding-mouth pancreas hypoplastic, abnormal
rpl23ahi2582Tg/hi2582Tg; jh1Tg; jh2Tg + MO4-tp53 standard conditions Protruding-mouth pancreas hypoplastic, abnormal
rpl6hi3655bTg/+; jh1Tg; jh2Tg + MO4-tp53 standard conditions Protruding-mouth pancreas hypoplastic, abnormal
rpl6hi3655bTg/hi3655bTg; jh1Tg; jh2Tg + MO4-tp53 standard conditions Protruding-mouth pancreas hypoplastic, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Zebrafish for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Zebrafish