ZFIN ID: ZDB-FIG-130719-28
Provost et al., 2013 - Multiple ribosomal proteins are expressed at high levels in developing zebrafish endoderm and are required for normal exocrine pancreas development. Zebrafish   10(2):161-169 Full text @ Zebrafish
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Anatomical Terms:
Stage Range: Prim-5 to Adult
PHENOTYPE:
Fish:
Observed In:
Stage: Protruding-mouth

Fig. 2 Mutations in the rpl23a gene result in compromised expansion of ptf1a-expressing pancreatic progenitor cells. (A) Expression of rpl23a during zebrafish development is broad at 24 hpf and becomes increasingly restricted to the larval endoderm during the course of development. (B) Strong expression of rpL23a is observed in adult liver, intestine, and pancreas. (C) An incross of rpl23ahi2582/ + ;ptf1a:eGFPjh1;ins:mCherry jh2 adult fish results in 72 hpf embryos exhibiting reduced brain and eye size (red arrowhead), edema, and poor yolk absorption (blue arrowhead). (D) Fraction of embryos from rpl23ahi2582/ + ;ptf1a:eGFPjh1;ins:mCherryjh2 incross displaying gross developmental defects as assessed at 72 hpf (29% mutant, red; 71%, wild type, blue) compared with control embryos generated by rpl23ahi2582/ + ;ptf1a:eGFPjh1;ins:mCherryjh2 outcross (2.8% mutant, red; 97.2% wild type, blue). (E) ptf1a-expressing pancreatic progenitor cells fail to expand normally in embryos generated by rpl23ahi2582/ + ;ptf1a:eGFPjh1;ins:mCherryjh2 incross. (F) Activation of trypsin expression occurs normally in rpl23ahi2582/hi2582 hypoplastic pancreas. Images in (E) and (F) obtained at 72 hpf. (G) PCR genotyping of embryos individually imaged at 72 hpf confirms reduced pancreatic mass in both heterozygous rpl23ahi2582/ + ;ptf1a:eGFPjh1; ins:mCherryjh2 and homozygous rpk23ahi2582/hi2582;ptf1a:eGFPjh1; ins:mCherryjh2 embryos. (H) Incidence of abnormal pancreas development in embryos produced by either incross or outcross rpl23ahi2582/ + ;ptf1a:eGFPjh1; ins:mCherryjh2 adults, expressed as the fraction of phenotypic (red) and wild-type (blue) embryos evaluated at 72 hpf. (I) Decreased incidence of persistent hypoplastic pancreas at 96 hpf indicates recovery of normal pancreatic mass in rpl23ahi2582/ + heterozygotes. Color images available online at www.liebertpub.com/zeb

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
rpl23a AB standard conditions Prim-5 whole organism ISH
Long-pec endodermal cell ISH
Long-pec intestine ISH
Long-pec liver ISH
Long-pec pancreatic bud ISH
Protruding-mouth endodermal cell ISH
Protruding-mouth intestine ISH
Protruding-mouth liver ISH
Protruding-mouth pancreas ISH
Day 5 endocrine pancreas ISH
Day 5 exocrine pancreas ISH
Adult epithelial cell of pancreas ISH
Adult hepatocyte ISH
Adult intestinal epithelial cell ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
rpl23ahi2582Tg/+; jh1Tg; jh2Tg standard conditions Protruding-mouth pancreas decreased volume, abnormal
Protruding-mouth pancreas development delayed, abnormal
rpl23ahi2582Tg/hi2582Tg; jh1Tg; jh2Tg standard conditions Protruding-mouth eye decreased size, abnormal
Protruding-mouth head decreased size, abnormal
Protruding-mouth pancreas decreased volume, abnormal
Protruding-mouth pancreas morphogenesis disrupted, abnormal
Protruding-mouth pericardium edematous, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Zebrafish for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Zebrafish