ZFIN ID: ZDB-FIG-130719-29
Provost et al., 2013 - Multiple ribosomal proteins are expressed at high levels in developing zebrafish endoderm and are required for normal exocrine pancreas development. Zebrafish   10(2):161-169 Full text @ Zebrafish
Anatomical Terms:
Stage Range: Prim-5 to Adult
Observed In:
Stage Range: Protruding-mouth to Adult

Fig. 3 Mutations in the rpl6 gene result in compromised expansion of ptf1a-expressing pancreatic progenitor cells. (A) rpl6 expression is initially broad at 24 hpf, but high-level expression becomes increasingly restricted to the larval endoderm during the course of development. (B) Strong expression of rpl6 is observed in adult liver and pancreas. (C) An incross of rpl6hi3655b/ + ;ptf1a:eGFPjh1;ins:mCherryjh2 adult fish results in 72 hpf embryos exhibiting reduced brain and eye size (green arrowhead), edema (red arrowhead), and poor yolk absorption (yellow arrowhead). Asterisks indicate grossly phenotypic rpl6hi3655b/hi3655b embryos. (D) Fraction of embryos from rpl6hi3655b/ + ;ptf1a:eGFPjh1;ins:mCherryjh2 incross displaying gross developmental defects as assessed at 72 hpf (21% mutant, red; 79% wild type, blue) compared with control embryos generated by rpl6hi3655b/ + ;ptf1a:eGFPjh1;ins:mCherryjh2 outcross (1.7% mutant, red; 98.3% wild type, blue). (E) The adult pancreas is grossly and histologically normal in rpl6hi3655b/ + ;ptf1a:eGFPjh1;ins:mCherryjh2 heterozygotes. Note normal gross appearance of 7- month-old heterozygote, grossly normal pancreas including acinar cell mass (green), principal islet (yellow arrowhead), and secondary islets (white arrowheads), as well as normal adult histology. (F) ptf1a-expressing pancreatic progenitor cells fail to expand normally in embryos generated by rpl6hi3655b/ + ;ptf1a:eGFPjh1;ins:mCherryjh2 incross. (G) Activation of trypsin expression occurs normally in rpl6hi3655b/hi3655b hypoplastic pancreas. (H) Incidence of abnormal pancreas development in embryos produced by either incross or outcross rpl6hi3655b/ + ;ptf1a:eGFPjh1; ins:mCherryjh2 adults, expressed as the fraction of phenotypic (red) and wild-type (blue) embryos evaluated at 72 hpf. (I) Decreased incidence of persistent hypoplastic pancreas at 96 hpf indicates recovery of normal pancreatic mass in rpl6hi3655b/ + heterozygotes. A, adipose tissue. Color images available online at www.liebertpub.com/zeb

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
rpl6 AB standard conditions Prim-5 whole organism ISH
Long-pec endodermal cell ISH
Long-pec intestine ISH
Long-pec liver ISH
Long-pec pancreas ISH
Protruding-mouth intestine ISH
Protruding-mouth liver ISH
Protruding-mouth pancreas ISH
Day 5 liver ISH
Day 5 pancreas ISH
Adult epithelial cell of pancreas ISH
Adult hepatocyte ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
rpl6hi3655bTg/+; jh1Tg; jh2Tg standard conditions Protruding-mouth pancreas decreased volume, abnormal
Protruding-mouth pancreas development delayed, abnormal
Protruding-mouth pancreas hypoplastic, abnormal
Adult pancreas morphology, normal
Adult pancreas volume, normal
rpl6hi3655bTg/hi3655bTg; jh1Tg; jh2Tg standard conditions Day 4 eye decreased size, abnormal
Day 4 head decreased size, abnormal
Day 4 pancreas decreased volume, abnormal
Day 4 pancreas development delayed, abnormal
Day 4 pancreas hypoplastic, abnormal
Day 4 pancreas morphogenesis disrupted, abnormal
Day 4 pericardium edematous, abnormal
ZFIN wishes to thank the journal Zebrafish for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Zebrafish