FIGURE

Fig. 6

ID

ZDB-FIG-130719-11

Publication

van der Vaart et al., 2013 - Functional analysis of a zebrafish myd88 mutant identifies key transcriptional components of the innate immune system

Other Figures

All Figure Page

Back to All Figure Page

Fig. 6

Bacterial .burdens of myd88^-/- and wild-type embryos infected with M. marinum. (A–F) Representative stereo fluorescence images of infected embryos. At 28 hpf, myd88^-/- (Mu) and wild-type (Wt) embryos were infected with <200 cfu of mCherry-labeled M. marinum strain Mma 20 (A,B) or strain E11 (C,D) by injection into the blood island. Stereo fluorescence images were taken at 3 (Mma20 strain) or 5 dpi (E11 strain). For comparison, wild-type and myd88-morpholino-injected embryos were infected with the same dose of M. marinum Mma 20 (E,F). (G–I) Quantification of bacterial burden. Red symbols indicate which infected individuals are shown as representative images in A–F. Bacterial pixel counts were determined based on stereo fluorescence images. Significant differences were determined by one-way ANOVA with Tukey’s Multiple Comparison method as a post-hoc test; ***P<0.001.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	myd88^{hu3568/hu3568} (AB) WT + MO2-myd88
Knockdown Reagent:	MO2-myd88
Observed In:	activation of innate immune response
Stage:	Day 5

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Dis. Model. Mech.