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ZFIN ID: ZDB-FIG-130619-9
Ignatius et al., 2013 - Distinct Functional and Temporal Requirements for Zebrafish Hdac1 during Neural Crest-Derived Craniofacial and Peripheral Neuron Development. PLoS One   8(5):e63218 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibody:
Fish:
Anatomical Terms:
Stage Range: Long-pec to Day 4
PHENOTYPE:
Fish:
Observed In:
Stage Range: Protruding-mouth to Day 4

Fig. 6 Sensory, enteric and sympathetic neuron development in hdac1b382 mutants.

A–D Anti-Hu/16A11 staining of wild-type and hdac1b382 mutants at 3dpf and 4dpf. Black arrowheads indicate normally positioned DRG in wild-type and hdac1b382 mutants. White arrowheads indicate examples of DRG neurons in ectopic locations. Black asterisks (A-D and inset) indicate the regions in the tail of wild-type and hdac1b382 mutants where DRG are present. White asterisks (B and inset, D) indicate the regions in the tail of hdac1b382 mutants where DRG are absent. E-G a cross sections of wild-type (E) and examples of 2 sections (F, G) of hdac1b382 mutants illustrating that DRG neurons in mutant embryos are often ectopically localized. H, I wild-type and hdac1b382 mutants with sox10 expression in the tail region at 52 hpf, black arrowheads in I indicate distinct foci of sox10 expression in mutant in locations where DRG would normally reside. J, K cross-sections of the anterior gut in wild-type and hdac1b382 mutants stained with anti-Hu/16A11 labeling enteric neurons at 4 dpf (white arrowheads). L, M th expression in the cervical sympathetic ganglion (black arrowhead) in wild-type and hdac1b382 mutants at 72 hpf. N, O, phox2b labeling of sympathetic neuron precursors (black arrowheads) in wild-type and hdac1b382 mutants at 56 hpf. P, Q, dbh staining of sympathetic neurons (black arrowheads) in wild-type and hdac1b382 mutants at 48 hpf. R, S, Anti Hu/16A11 staining of wild-type and hdac1b382 mutants at 3dpf with black arrowheads indicating sympathetic neurons.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
dbh WT standard conditions Long-pec sympathetic neuron ISH
hdac1b382/b382 standard conditions Long-pec sympathetic neuron ISH
phox2bb WT standard conditions Long-pec brain ISH
Long-pec sympathetic neuron ISH
hdac1b382/b382 standard conditions Long-pec brain ISH
Long-pec sympathetic neuron ISH
sox10 WT standard conditions Long-pec dorsal root ganglion ISH
hdac1b382/b382 standard conditions Long-pec dorsal root ganglion ISH
th WT standard conditions Protruding-mouth brain ISH
Protruding-mouth superior cervical ganglion ISH
hdac1b382/b382 standard conditions Protruding-mouth brain ISH
Protruding-mouth superior cervical ganglion ISH
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-elavl IHC WT standard conditions Protruding-mouth dorsal root ganglion
IHC Protruding-mouth sympathetic neuron
IHC Day 4 dorsal root ganglion
IHC Day 4 enteric neuron
IHC hdac1b382/b382 standard conditions Protruding-mouth dorsal root ganglion
IHC Protruding-mouth sympathetic neuron
IHC Day 4 dorsal root ganglion
IHC Day 4 enteric neuron
Phenotype Details
Fish Conditions Stage Phenotype
hdac1b382/b382 standard conditions Protruding-mouth caudal fin lacks parts or has fewer parts of type dorsal root ganglion, abnormal
Protruding-mouth dorsal root ganglion mislocalised, abnormal
Day 4 caudal fin lacks parts or has fewer parts of type dorsal root ganglion, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One