FIGURE

Fig. 1

ID

ZDB-FIG-130619-37

Publication

Ito et al., 2013 - Close Association of Carbonic Anhydrase (CA2a and CA15a), Na(+)/H(+) Exchanger (Nhe3b), and Ammonia Transporter Rhcg1 in Zebrafish Ionocytes Responsible for Na(+) Uptake

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Fig. 1

Expression of ca2a and ca15a mRNA and their protein products in H-MRC. Zebrafish larvae of 55-hpf were probed for expression of the indicated genes (A,D) and their products (G,J), and the types of positive cells were identified by costaining with anti-H⁺-ATPase (B,E,H) and concanavalin A [ConA, (K)], which are markers for H-MRC. Right panels (C,F,I,L) are merged images. All panels show lateral views of dorsal trunk and yolk sac extension of specimens oriented dorsal side up, anterior to the left. Large (30-50 µm) dendritic cells of dark brown in (A,C,D,F) are melanocytes. Arrowheads in (A-F) point to typical H-MRCs coexpressing ca2a, ca15a, and H⁺-ATPase. Arrowheads in (G,J-L) indicate the apical pits of H-MRC. Arrows in (H) show donut-like staining surrounding the pits.

Expression Data

Genes:	ca2 ca15a slc9a3.2
Antibodies:	Ab1-ca2 Ab1-ca15a Ab1-slc9a3.2
Fish:	TL
Anatomical Term:	vH ionocyte
Stage Range:	Long-pec to Days 7-13

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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