ZFIN ID: ZDB-FIG-130523-1
Chen et al., 2013 - Snx3 Regulates Recycling of the Transferrin Receptor and Iron Assimilation. Cell Metabolism   17(3):343-352 Full text @ Cell Metab.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Term:
Stage: Prim-5
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage Range: Long-pec to Protruding-mouth

Fig. 2

Snx3 Is Required for Heme Production in Zebrafish Embryos

(A) Whole-mount in situ hybridization detects expression of gata1 and snx3 in the blood island ICM in zebrafish embryos at 24 hpf. The ICM staining is absent in cloche mutants and is expanded in dino mutants.

(B) Western analysis confirms efficient silencing of snx3 by a translation-blocking morpholino (MO1) and a pre-mRNA splice-blocking morpholino (MO2). The expression of snx4 and ±-actin control proteins was unaffected.

(C) Injections of MOs against snx3 result in a profound anemia in zebrafish embryos at both 48 and 72 hpf.

(D) Knockdown of snx3 in Tg(globin-LCR:eGFP) transgenic zebrafish leads to reduced number of erythrocytes at 72 hpf, but not at 48 hpf. Error bars represent SEM from three biological replicates. *p < 0.001 compared with the control morpholino.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
gata1a AB control Prim-5 blood island ISH
chrdtm84/tm84 standard conditions Prim-5 blood island ISH
npas4lDf(Chr13:lycat)m39(INDO) standard conditions Prim-5 Not Detected blood island ISH
snx3 AB control Prim-5 blood island ISH
chrdtm84/tm84 standard conditions Prim-5 blood island ISH
npas4lDf(Chr13:lycat)m39(INDO) standard conditions Prim-5 Not Detected blood island ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
AB + MO1-snx3 standard conditions Long-pec whole organism lacks parts or has fewer parts of type nucleate erythrocyte hemoglobin complex, abnormal
Protruding-mouth whole organism lacks parts or has fewer parts of type nucleate erythrocyte hemoglobin complex, abnormal
AB + MO2-snx3 standard conditions Long-pec whole organism lacks parts or has fewer parts of type nucleate erythrocyte hemoglobin complex, abnormal
Protruding-mouth whole organism lacks parts or has fewer parts of type nucleate erythrocyte hemoglobin complex, abnormal
cz3325Tg + MO2-snx3 standard conditions Protruding-mouth nucleate erythrocyte decreased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Cell Metabolism for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Cell Metabolism, 17(3), Chen, C., Garcia-Santos, D., Ishikawa, Y., Seguin, A., Li, L., Fegan, K.H., Hildick-Smith, G.J., Shah, D.I., Cooney, J.D., Chen, W., King, M.J., Yien, Y.Y., Schultz, I.J., Anderson, H., Dalton, A.J., Freedman, M.L., Kingsley, P.D., Palis, J., Hattangadi, S.M., Lodish, H.F., Ward, D.M., Kaplan, J., Maeda, T., Ponka, P., and Paw, B.H., Snx3 Regulates Recycling of the Transferrin Receptor and Iron Assimilation, 343-352, Copyright (2013) with permission from Elsevier. Full text @ Cell Metab.