|ZFIN ID: ZDB-IMAGE-161028-39|
Snx3 Is Required for Heme Production in Zebrafish Embryos
(A) Whole-mount in situ hybridization detects expression of gata1 and snx3 in the blood island ICM in zebrafish embryos at 24 hpf. The ICM staining is absent in cloche mutants and is expanded in dino mutants.
(B) Western analysis confirms efficient silencing of snx3 by a translation-blocking morpholino (MO1) and a pre-mRNA splice-blocking morpholino (MO2). The expression of snx4 and ±-actin control proteins was unaffected.
(C) Injections of MOs against snx3 result in a profound anemia in zebrafish embryos at both 48 and 72 hpf.
(D) Knockdown of snx3 in Tg(globin-LCR:eGFP) transgenic zebrafish leads to reduced number of erythrocytes at 72 hpf, but not at 48 hpf. Error bars represent SEM from three biological replicates. *p < 0.001 compared with the control morpholino.
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