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ZFIN ID: ZDB-FIG-130516-16
O'Neill et al., 2013 - BMP signaling and spadetailregulate exit of muscle precursors from the zebrafish tailbud. Developmental Biology   375(2):117-127 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibody:
Fish:
Condition:
Knockdown Reagents:
Anatomical Terms:
Stage Range: 14-19 somites to Prim-5
PHENOTYPE:
Fish:
Condition:
Knockdown Reagents:
Observed In:
Stage Range: 14-19 somites to Prim-5

Fig. 3 BMP inhibition is required for cells to exit the tailbud and form somites. P-smad 1/5/8 antibody was used to detect BMP activity in the tailbud of embryos during somitiogenesis. (A)–(D) dorsal view of P-smad 1/5/8 antibody fluorescence in 17 hpf (16 som) embryos; (E)–(G) are lateral views of P-smad 1/5/8 staining in 17 hpf embryos. WT embryos have normal levels of active BMP ((A) and (E)). Chd-/- (B) and oepMO (F) embryos have elevated levels of BMP, possibly due to the roles of chd and oep in BMP inhibition. chd-/-;spt-/- (D) and oepMO;sptMO (G) embryos also have higher levels of BMP in the tailbud, however, elevated levels are found in only some areas of the tailbud. Elevated Bmp activity and lack of spt leads to phenotypes where progenitor cells cannot exit the tailbud to form somites. (H)–(I), dorsal view of myoD expression in 24 hpf embryos: chd;spt embryos treated with dorsomorphin, a small molecule inhibitor of BMP, at 12 hpf were partially rescued and able to produce tail somites. (23/25 had 5–9 somites) (I), whereas untreated chd;spt embryos do not produce somites (1/26 had 5–9 somites) (H).

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
myod1 tbx16b104/b104; chrdks1/ks1 standard conditions Prim-5 Not Detected post-vent region somite ISH
tbx16b104/b104; chrdks1/ks1 chemical treatment: pharmaceutical Prim-5 post-vent region somite ISH
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-smad IHC WT standard conditions 14-19 somites tail bud
IHC WT + MO1-tdgf1 standard conditions 14-19 somites tail bud
IHC WT + MO1-tdgf1 + MO4-tp53 standard conditions 14-19 somites tail bud
IHC chrdks1/ks1 standard conditions 14-19 somites tail bud
IHC tbx16b104/b104 standard conditions 14-19 somites tail bud
IHC tbx16b104/b104; chrdks1/ks1 standard conditions 14-19 somites tail bud
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-tdgf1 standard conditions 14-19 somites tail bud increased size, abnormal
WT + MO1-tdgf1 + MO4-tp53 standard conditions 14-19 somites tail bud increased size, abnormal
chrdks1/ks1 standard conditions 14-19 somites tail bud increased size, abnormal
tbx16b104/b104 standard conditions 14-19 somites tail bud increased size, abnormal
tbx16b104/b104; chrdks1/ks1 standard conditions 14-19 somites tail bud increased size, abnormal
Prim-5 somitogenesis disrupted, abnormal
Prim-5 tail bud increased size, abnormal
tbx16b104/b104; chrdks1/ks1 chemical treatment: pharmaceutical Prim-5 somitogenesis present, normal
Prim-5 tail bud increased size, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 375(2), O'Neill, K., and Thorpe, C., BMP signaling and spadetailregulate exit of muscle precursors from the zebrafish tailbud, 117-127, Copyright (2013) with permission from Elsevier. Full text @ Dev. Biol.