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ZFIN ID: ZDB-FIG-130516-15
O'Neill et al., 2013 - BMP signaling and spadetailregulate exit of muscle precursors from the zebrafish tailbud. Developmental Biology   375(2):117-127 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: 1-4 somites to Prim-5
PHENOTYPE:
Fish:
Observed In:
Stage Range: 1-4 somites to Prim-5

Fig. 2 Chd;spt mutants produce mesodermal progenitor cells in the tailbud but are not able to form somites. (A)–(D) Dorsal view of myoD expression in 24 hpf embryos. (A)–(C) WT, chd, and spt embryos form organized somites even if it is only in the tail (spt). chd;spt mutants do not form organized somites. (60/65 have no organized somites. The remaining 5 only had 2–3 somites form.) (D). (E)–(H), dorsal view of ntn1b expressing muscle cells and tbx6 expressing MPCs in 11 hpf embryos. (E)–(G) WT, chd, and spt embryos have MPCs in tailbud as well as differentiated muscle cells outside the tailbud. chd;spt embryos have an accumulation of MPCs in the tailbud and no differentiated muscle cells. (I)–(L), lateral view of papc expression in 24 hpf embryos. WT and mutant embryos have MPCs in the tailbud with spt and chd;spt embryos having a large accumulation of progenitor cells.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
myod1 WT standard conditions Prim-5 myotome ISH
chrdks1/ks1 standard conditions Prim-5 myotome ISH
tbx16b104/b104 standard conditions Prim-5 myotome ISH
tbx16b104/b104; chrdks1/ks1 standard conditions Prim-5 trunk muscle cell ISH
ntn1b WT standard conditions 1-4 somites trunk muscle cell ISH
chrdks1/ks1 standard conditions 1-4 somites trunk muscle cell ISH
tbx16b104/b104 standard conditions 1-4 somites trunk muscle cell ISH
tbx16b104/b104; chrdks1/ks1 standard conditions 1-4 somites Not Detected trunk ISH
pcdh8 WT standard conditions Prim-5 tail bud myoblast ISH
chrdks1/ks1 standard conditions Prim-5 tail bud myoblast ISH
tbx16b104/b104 standard conditions Prim-5 tail bud myoblast ISH
tbx16b104/b104; chrdks1/ks1 standard conditions Prim-5 tail bud myoblast ISH
tbx6 WT standard conditions 1-4 somites tail bud myoblast ISH
chrdks1/ks1 standard conditions 1-4 somites tail bud myoblast ISH
tbx16b104/b104 standard conditions 1-4 somites tail bud myoblast ISH
tbx16b104/b104; chrdks1/ks1 standard conditions 1-4 somites tail bud myoblast ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
chrdks1/ks1 standard conditions 1-4 somites post-vent region somite decreased size, abnormal
chrdks1/ks1 standard conditions 1-4 somites tail bud increased size, abnormal
chrdks1/ks1 standard conditions 1-4 somites trunk somite decreased size, abnormal
chrdks1/ks1 standard conditions Prim-5 tail bud increased size, abnormal
chrdks1/ks1 standard conditions Prim-5 trunk somite decreased size, abnormal
tbx16b104/b104 standard conditions 1-4 somites post-vent region somite decreased size, abnormal
1-4 somites tail bud increased size, abnormal
1-4 somites trunk somite decreased size, abnormal
Prim-5 tail bud increased size, abnormal
Prim-5 trunk somite decreased size, abnormal
tbx16b104/b104; chrdks1/ks1 standard conditions 1-4 somites tail bud increased size, abnormal
1-4 somites whole organism somite absent, abnormal
Prim-5 tail bud increased size, abnormal
Prim-5 whole organism somite absent, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 375(2), O'Neill, K., and Thorpe, C., BMP signaling and spadetailregulate exit of muscle precursors from the zebrafish tailbud, 117-127, Copyright (2013) with permission from Elsevier. Full text @ Dev. Biol.