ZFIN ID: ZDB-FIG-130419-4
Rahn et al., 2013 - Opa1 is required for proper mitochondrial metabolism in early development. PLoS One   8(3):e59218 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Term:
Stage Range: Prim-5 to Day 4

Fig. 5

Gene expression changes of mitochondrial morphology genes in MMC morphants (black) and Opa1 morphants (grey) normalized to MMC morphant levels.

Significant increases in gene expression of mitochondrial fusion proteins (A) opa1 (a, p = 0.003), (B) mfn1 (b, p = 0.001) and (C) mfn2 (c, p = 0.01) were observed in Opa1 morphants compared to MMC morphants. No differences were observed for (D) drp1, a mitochondrial fission protein. Error bars are shown +/- SEM, n = 5. P-values obtained by ANOVA.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
dnm1l AB control Prim-5 to Day 4 whole organism RTPCR
AB + MO1-opa1 standard conditions Prim-5 to Day 4 whole organism RTPCR
mfn1b AB control Prim-5 to Day 4 whole organism RTPCR
AB + MO1-opa1 standard conditions Prim-5 to Day 4 whole organism RTPCR
mfn2 AB control Prim-5 to Day 4 whole organism RTPCR
AB + MO1-opa1 standard conditions Prim-5 to Day 4 whole organism RTPCR
opa1 AB control Prim-5 to Day 4 whole organism RTPCR
AB + MO1-opa1 standard conditions Prim-5 to Day 4 whole organism RTPCR
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One