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ZIRC
ZFIN ID: ZDB-FIG-130311-20
Ye et al., 2013 - S1pr2/Gα13 signaling controls myocardial migration by regulating endoderm convergence. Development (Cambridge, England)   140(4):789-799 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage Range: 20-25 somites to Prim-15
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Prim-15

Fig. 1 Gα13 signaling is required for myocardial migration. (A-F) Comparison of control embryos (A,B), embryos injected with MOs against both gna13a and gna13b (C,D, gna13ab MOs), and embryos injected with a MO against s1pr2/mil (E,F) at 35 hpf. (A,C,E) Lateral bright-field images of embryos. Anterior is towards the left; arrows indicate pericardial edema; arrowheads indicate tail blisters. (A′,C′,E′) High-magnification images of the tail region of each embryo. (B,D,F) Overlay of epifluorescence and bright-field images of Tg(myl7:EGFP) embryos at 30 hpf. Ventral view; white arrows indicate hearts. (G-J) Expression of nkx2.5 and myl7, as detected by whole-mount in situ hybridization, in control and gnb13ab MO-injected embryos. Dorsoanterior view with anterior upwards. (K) Frequencies of cardia bifida and tail blistering in 35 hpf embryos injected with gna13ab MOs, alone or together with an RNA encoding the human Gα13 (GNA13). *P<0.05; **P<0.01. Data are mean±s.e.m. Scale bars: 100 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP twu34Tg control Prim-15 heart tube IFL
twu34Tg + MO1-gna13a + MO1-gna13b standard conditions Prim-15 heart tube IFL
twu34Tg + MO1-s1pr2 standard conditions Prim-15 heart tube IFL
myl7 WT control Prim-15 heart tube ISH
Prim-15 myocardium ISH
WT + MO1-gna13a + MO1-gna13b standard conditions Prim-15 heart tube ISH
Prim-15 myocardium ISH
nkx2.5 WT control 20-25 somites heart rudiment ISH
20-25 somites myocardium ISH
WT + MO1-gna13a + MO1-gna13b standard conditions 20-25 somites heart rudiment ISH
20-25 somites myocardium ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-gna13a + MO1-gna13b standard conditions Prim-15 caudal fin blistered, abnormal
Prim-15 pericardium edematous, abnormal
WT + MO1-s1pr2 standard conditions Prim-15 caudal fin blistered, abnormal
Prim-15 pericardium edematous, abnormal
twu34Tg + MO1-gna13a + MO1-gna13b standard conditions Prim-15 heart duplicated, abnormal
twu34Tg + MO1-s1pr2 standard conditions Prim-15 heart duplicated, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development