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ZFIN ID: ZDB-FIG-130311-18
Ye et al., 2013 - S1pr2/Gα13 signaling controls myocardial migration by regulating endoderm convergence. Development (Cambridge, England)   140(4):789-799 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Term:
Stage: Prim-15
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Prim-15

Fig. S1 12 and Gα13 have partially redundant roles in myocardial migration. Tg(myl7:EGFP) embryos injected with gnb13ab MOs (1 ng each) and gnb12 MO (4 ng), alone or in combination, at 30 hpf. (A-C) Overlay of epifluorescence and bright-field images. Ventral view; white arrows indicate hearts. (D-F) myl7 expression, as detected by in situ hybridization. Dorsoanterior view with anterior upwards. (G) Frequencies of indicated embryos that exhibit cardia bifida at 35 hpf. Scale bars: 100 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP twu34Tg + MO1-gna12a standard conditions Prim-15 cardiac muscle cell IFL
twu34Tg + MO1-gna12a + MO1-gna13a + MO1-gna13b standard conditions Prim-15 cardiac muscle cell IFL
twu34Tg + MO1-gna13a + MO1-gna13b standard conditions Prim-15 cardiac muscle cell IFL
myl7 WT + MO1-gna12a standard conditions Prim-15 cardiac muscle cell ISH
WT + MO1-gna12a + MO1-gna13a + MO1-gna13b standard conditions Prim-15 cardiac muscle cell ISH
WT + MO1-gna13a + MO1-gna13b standard conditions Prim-15 cardiac muscle cell ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-gna12a + MO1-gna13a + MO1-gna13b standard conditions Prim-15 heart duplicated, abnormal
WT + MO1-gna13a + MO1-gna13b standard conditions Prim-15 heart duplicated, abnormal
twu34Tg + MO1-gna12a + MO1-gna13a + MO1-gna13b standard conditions Prim-15 heart duplicated, abnormal
twu34Tg + MO1-gna13a + MO1-gna13b standard conditions Prim-15 heart duplicated, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development