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ZIRC
ZFIN ID: ZDB-FIG-130311-27
Ye et al., 2013 - S1pr2/Gα13 signaling controls myocardial migration by regulating endoderm convergence. Development (Cambridge, England)   140(4):789-799 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage: Prim-5
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Prim-5

Fig. 7 Disrupting the S1pr2/Gα13/RhoGEF signaling pathway causes defects in endoderm morphogenesis, as well as in myocardial migration. (A-F) Epifluorescence images of the anterior regions of the endoderm in 26 hpf, double-transgenic Tg(sox17:EGFP)/(myl7:EGFP) embryos. (A) Control uninjected siblings. (B-F) Embryos injected with: the gnb13ab MOs alone (B); the gnb13ab MOs plus the GNA13 RNA (C); the GNA13 RNA alone (D); the s1pr2 MO (E); and the Arhgef11RGS RNA (F). Dorsoanterior views with anterior upwards; yellow dots indicate cardiomyocytes; yellow lines (same length) indicate the width of the anterior endodermal sheet; blue asterisks indicate endodermal holes. (G) A proposed model for how S1pr2 regulates myocardial migration (see text for details). (H) Frequencies of incidences of endodermal holes and cardia bifida in 26 hpf embryos injected with the indicated MOs and RNAs. (I) Endodermal width and the distance between two populations of cardiomyocytes in 26 hpf embryos (obtained from the same pair of fish) injected with the indicated MOs and RNAs. *P< 0.01 versus control; **P<0.01 versus gna13ab MOs; #P>0.05 versus control. Data are mean±s.e.m. Scale bar: 100 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP ha01Tg; twu34Tg control Prim-5 cardiac muscle cell IFL
Prim-5 endodermal cell IFL
ha01Tg; twu34Tg + MO1-gna13a + MO1-gna13b standard conditions Prim-5 cardiac muscle cell IFL
Prim-5 endodermal cell IFL
ha01Tg; twu34Tg + MO1-s1pr2 standard conditions Prim-5 cardiac muscle cell IFL
Prim-5 endodermal cell IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
ha01Tg; twu34Tg + MO1-gna13a + MO1-gna13b standard conditions Prim-5 endoderm anterior region increased width, abnormal
Prim-5 endoderm anterior region perforate, abnormal
Prim-5 heart duplicated, abnormal
ha01Tg; twu34Tg + MO1-s1pr2 standard conditions Prim-5 endoderm anterior region increased width, abnormal
Prim-5 endoderm anterior region perforate, abnormal
Prim-5 heart duplicated, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development