FIGURE

Fig. 3

ID

ZDB-FIG-130304-14

Publication

Lackner et al., 2013 - Segmental assembly of fibronectin matrix requires rap1b and integrin alpha5

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Fig. 3

Loss of somite borders is likely not due to patterning defects in the PSM. A-E: Double in situ hybridization for myoD (pink) and her1 (blue) for (A) wild-type, (B) rap1b MO1, (C) rap1b DN mRNA, (D) itgα5^-/-; rap1b MO1, and (E) itgα5^-/-; rap1b DN 12-15-somite-stage embryos. Stripes of her1 expression persist in all backgrounds. In contrast, segmental myoD expression is blurred in rap1b MO1 and rap1b DN embryos, and completely lost in itgα5^-/-; rap1b MO or itgα5^-/-; rap1b DN. F-H: Double in situ hybridization for myoD (pink) and mesp-b (blue) in (F) wild-type, (G) rap1b MO1, and (H) itgα5^-/-; rap1b MO1 embryos at 12-15-somite stage. Stripes of mesp-b can be seen in both rap1b and itgα5^-/-; rap1b morphants. I-K: In situ hybridization for ripply1 in (I) wild-type, (J) rap1b MO1, and (K) itgα5^-/-; rap1b MO1 12-15-somite stage embryos. Segmental ripply1 expression is disrupted only in itgα5^-/-; rap1b MO1 embryos. For all images, anterior is up.

Expression Data

Genes:	her1 mespba myod1 ripply1
Fish:	WT WT + MO1-rap1b itga5^tbfe1/tbfe1 + MO1-rap1b itga5^tbfe2/tbfe2 + MO1-rap1b
Knockdown Reagent:	MO1-rap1b
Anatomical Terms:	segmental plate somite
Stage Range:	10-13 somites to 14-19 somites

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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