FIGURE

Fig. 1

ID

ZDB-FIG-130304-12

Publication

Lackner et al., 2013 - Segmental assembly of fibronectin matrix requires rap1b and integrin alpha5

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Fig. 1

Loss of rap1b synergizes with itgα5, resulting in a disruption of all somite borders and abrogation of Fibronectin matrix assembly. A?F: Lateral view DIC images showing clear somite borders for (A) wild-type, (B) rap1b MO1-injected (8 experiments, 83.7% affected, n = 341), and (C) rap1b DN mRNA-injected (3 experiments, 100% affected, n = 177) embryos. For synergy experiments, rap1b MO1 or rap1b DN was injected into the progeny of heterozygous itgα5^+/- crosses. Therefore, 100% penetrance would result in approximately 25% of embryos exhibiting the phenotype. D: Anterior somite borders are disrupted in the itgα5^-/- mutant, while (E) itgα5^-/-; rap1b MO1 (9 experiments, 27.4% affected, n = 865) and (F) itgα5^-/-; rap1b DN (6 experiments, 22.3% affected, n = 455) embryos lack somite borders along the entire AP axis. (G- L) Dorsal view and (G′-L′) higher magnification dorsal view of FN localization showing clear FN matrix surrounding somites in (G?G′) wild-type, (H?H′) rap1b MO1, and (I?I′) rap1b DN embryos. J,J′: FN matrix surrounds only posterior somites in itgα5^-/- mutants, but few fibrils are seen within the paraxial mesoderm of either (K,K′) itgα5^-/-; rap1b MO1 or (L,L′) itgα5^-/-; rap1b DN embryos. For all images, anterior is to the left and embryos are at the 12?15-somite stage.

Expression Data

Antibody:	Ab2-fn
Fish:	WT itga5^tbfe1/tbfe1 itga5^tbfe2/tbfe2 WT + MO1-rap1b itga5^tbfe1/tbfe1 + MO1-rap1b itga5^tbfe2/tbfe2 + MO1-rap1b
Knockdown Reagent:	MO1-rap1b
Anatomical Terms:	notochord paraxial mesoderm somite somite anatomical region
Stage Range:	10-13 somites to 14-19 somites

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	itga5^tbfe1/tbfe1 itga5^tbfe2/tbfe2 WT + MO1-rap1b itga5^tbfe1/tbfe1 + MO1-rap1b itga5^tbfe2/tbfe2 + MO1-rap1b
Knockdown Reagent:	MO1-rap1b
Observed In:	caudal fin cell somite somite border tail bud dorsal side whole organism whole organism anterior-posterior axis
Stage Range:	10-13 somites to 14-19 somites

Phenotype Detail

Acknowledgments

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