FIGURE

Fig. 5

ID

ZDB-FIG-130304-16

Publication

Lackner et al., 2013 - Segmental assembly of fibronectin matrix requires rap1b and integrin alpha5

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Fig. 5

Somite morphogenesis is sensitive to genetic interaction between itgα5 and ephrinB2a, but not rap1b and ephrinB2a. A-C: Lateral view DIC images showing complete disruption of somite borders in (A) itgα5^-/-; ephrinB2a MO embryos (7 experiments, 27.9% affected compared to an expected 25% for homozygotes, n = 605) and proper maintenance of somite borders in (B) ephrinB2a MO + rap1b MO1 (5 experiments, no synergy, n = 455) and (C) ephrinB2a MO + rap1b DN embryos (3 experiments, no synergy). Dorsal (D-F:) and higher magnification dorsal (D′-F′) views of Fibronectin localization for (D-D′) itgα5^-/-; ephrinB2a MO, (E-E′) ephrinB2a MO + rap1b MO1, and (F-F′) ephrinB2a MO + rap1b DN embryos at the 12–15-somite stage. FN matrix formation is lost only in itgα5^-/-; ephrinB2a MO embryos. For all images, anterior is left.

Expression Data

Antibody:	Ab2-fn
Fish:	itga5^tbfe1/tbfe1 + MO2-efnb2a itga5^tbfe2/tbfe2 + MO2-efnb2a WT + MO1-rap1b + MO2-efnb2a
Knockdown Reagents:	MO1-rap1b MO2-efnb2a
Anatomical Terms:	somite somite anatomical region
Stage Range:	10-13 somites to 14-19 somites

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	itga5^tbfe1/tbfe1 + MO2-efnb2a itga5^tbfe2/tbfe2 + MO2-efnb2a
Knockdown Reagent:	MO2-efnb2a
Observed In:	somite border
Stage Range:	10-13 somites to 14-19 somites

Phenotype Detail

Acknowledgments

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