ZFIN ID: ZDB-FIG-130225-7
Dong et al., 2012 - Suppression of rap1 impairs cardiac myofibrils and conduction system in zebrafish. PLoS One   7(11):e50960 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage Range: 14-19 somites to Prim-15
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage Range: 14-19 somites to Prim-15

Fig. 2 Time-lapsed observation revealed abnormal heart development in Rap1 knock-down zebrafish.

Transgenic fish mp157e without any injection (A) and injected rap1MO (A′) were indistinguishable in early heart development. By 24 hpf, rap1MO injected mp157e embryos, crossed in flk1:mCherry background, started to show abnormality in heart development and remodeling (B to B′), including the failure of heart tube extension, abnormal cardiac looping and incomplete heart chamber formation. In higher magnification views, the morphants show that a small portion of GFP labeled cardiac cells (arrows in B′ to D′) did not migrate properly to be eventually packed in the heart tube. Dorsal view, head to left in A to C and A′ to C′. Lateral view, head to left in B to D and B′ to D′. Scale bar, 100 µm in A to C, and A′ to C′; 40 µm in D and D′.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP pku5Et; pku6Tg control 14-19 somites cardiac muscle cell IFL
Prim-5 cardiac muscle cell IFL
Prim-15 cardiac muscle cell IFL
pku5Et; pku6Tg + MO1-rap1aa + MO2-rap1b + MO4-tp53 standard conditions 14-19 somites cardiac muscle cell IFL
Prim-5 cardiac muscle cell IFL
Prim-15 cardiac muscle cell IFL
mCherry pku5Et; pku6Tg control Prim-15 blood vessel IFL
pku5Et; pku6Tg + MO1-rap1aa + MO2-rap1b + MO4-tp53 standard conditions Prim-15 blood vessel IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
pku5Et + MO1-rap1aa + MO2-rap1b + MO4-tp53 standard conditions 14-19 somites heart primordium morphology, normal
Prim-5 heart development disrupted, abnormal
pku5Et; pku6Tg + MO1-rap1aa + MO2-rap1b + MO4-tp53 standard conditions Prim-15 heart development disrupted, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS One