FIGURE

Fig. 5

ID

ZDB-FIG-130208-21

Publication

Monk et al., 2013 - Mutation of sec63 in zebrafish causes defects in myelinated axons and liver pathology

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Fig. 5

Markers of ER stress are elevated in sec63^st67 mutants. (A) RT-PCR showing expression of spliced and unspliced xbp-1 in individual larvae of the indicated genotypes and developmental stages. During the UPR, a 26-nucleotide fragment of xbp-1 mRNA is spliced. RT-PCR shows that the spliced form of xbp-1 (S) is enriched in sec63^st67 mutants compared with wild-type (WT) and st67/+ larvae. Unspliced xbp-1 (US) is present in all samples. As a positive control for the assay, wild-type larvae treated with the ER stressor tunicamycin show upregulation of the spliced form of xbp-1. (B,C) qRT-PCR showing relative expression of UPR markers bip (B) and chop (C) in wild-type and heterozygous siblings (WT) vs sec63^st67 mutant larvae (st67) at the indicated stages. Error bars indicate s.d.

Expression Data

Genes:	ddit3 hspa5 xbp1
Fish:	WT sec63^st67/st67
Anatomical Term:	whole organism
Stage Range:	Prim-5 to Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	sec63^st67/st67
Observed In:	endoplasmic reticulum unfolded protein response
Stage Range:	Protruding-mouth to Day 5

Phenotype Detail

Acknowledgments

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