ZFIN ID: ZDB-FIG-130128-43
Garnaas et al., 2012 - Rargb regulates organ laterality in a zebrafish model of right atrial isomerism. Developmental Biology   372(2):178-189 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Knockdown Reagents:
Anatomical Term:
Stage: Protruding-mouth
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Protruding-mouth

Fig. 3 Retinoic acid receptors differentially impact liver development and laterality. (A–L) Lfabp expression at 72 hpf in RAR morphant embryos. Loss of RARs raraa, rarab, and rarga leads to a reduction in liver size (A–D and G–J), whereas loss of rargb causes embryos to develop bilateral livers (E, K and K2). Embryos simultaneously injected with a lower dose of all four RAR MOs develop small livers or none at all (F and L). (M–P) Embryos treated with the RARγ antagonist MM11253 over the time points given develop bilateral livers, confirming genetic knockdown data. (Q) FACS quantification of percent GFP+ cells in lfabp:GFP RAR morphant embryos reveals that rargb morphant embryos with bilateral livers contain significantly more GFP+ hepatocytes than control siblings. Embryos injected with raraa, rarab, rarga, and rargb in combination have roughly half as many GFP+ hepatocytes as control embryos. Scale bars: 100 µm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
fabp10a WT control Protruding-mouth liver ISH
WT + MO1-raraa standard conditions Protruding-mouth liver ISH
WT + MO1-rarab standard conditions Protruding-mouth liver ISH
WT + MO1-rarga standard conditions Protruding-mouth liver ISH
WT + MO1-rargb standard conditions Protruding-mouth liver ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-raraa standard conditions Protruding-mouth liver decreased size, abnormal
WT + MO1-raraa + MO1-rarab + MO1-rarga + MO1-rargb standard conditions Protruding-mouth liver absent, abnormal
Protruding-mouth liver decreased size, abnormal
WT + MO1-rarab standard conditions Protruding-mouth liver decreased size, abnormal
WT + MO1-rarga standard conditions Protruding-mouth liver decreased size, abnormal
WT + MO1-rargb standard conditions Protruding-mouth liver bilateral, abnormal
zf235Tg + MO1-raraa standard conditions Protruding-mouth hepatocyte decreased amount, abnormal
zf235Tg + MO1-raraa + MO1-rarab + MO1-rarga + MO1-rargb standard conditions Protruding-mouth hepatocyte decreased amount, abnormal
zf235Tg + MO1-rargb standard conditions Protruding-mouth hepatocyte increased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 372(2), Garnaas, M.K., Cutting, C.C., Meyers, A., Kelsey, P.B., Harris, J.M., North, T.E., and Goessling, W., Rargb regulates organ laterality in a zebrafish model of right atrial isomerism, 178-189, Copyright (2012) with permission from Elsevier. Full text @ Dev. Biol.