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ZIRC
ZFIN ID: ZDB-FIG-121217-32
Nguyen-Chi et al., 2012 - Morphogenesis and Cell Fate Determination within the Adaxial Cell Equivalence Group of the Zebrafish Myotome. PLoS Genetics   8(10):e1003014 Full text @ PLoS Genet.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibodies:
Fish:
Condition:
Anatomical Terms:
Stage Range: 5-9 somites to Prim-5
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage: Prim-5

Fig. 5 sprouty4 loss-of-function leads to the reduction of MP formation.

(A) Sequencing of spry4WT and spry4fh117 confirms the single A to T mutation in position 469 of the spry4 ORF, resulting in the premature stop codon. (B) Predicted peptide arising from spry4WT and spry4fh117. The mutation results in a truncated protein of 157 amino acids (K157 in red is replaced by a stop codon) lacking the conserved cysteine rich domain [31] also called the spry domain (blue). This domain has been involved in RTK inhibition in other systems. (C–F) spry4 expression at 10-somite in WT, spry4fh117/+, Z spry4fh117 and MZ spry4fh117 embryos as determined by in situ hybridization. Whole mounted embryos, lateral view. (G–H) Erm expression in 9-somite WT and MZ spry4fh117 embryos. Flat mounted embryos, anterior toward the top. (I–J) sMyhc expression (red) in the somites of (I) WT and (J) MZ spry4fh117 embryos at 1 dpf. Maximal projection of multiple confocal scans, lateral view. (K) Graphic representation of the number of MPs per somite of 1 dpf embryos in different conditions: WT, spry4fh117/+, and MZ spry4fh117 untreated embryos or after DMSO or SU5402 treatments, values = means, error bars = s.e.m, ***p<0.001. (L–O) Engrailed (green) and Prox1 (red) expression in the somites of 1 dpf WT or MZ spry4fh117 embryos, after DMSO or SU5402 treatments, scale bar = 50 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
etv5b WT standard conditions 5-9 somites somite ISH
spry4fh117/fh117 standard conditions 5-9 somites somite ISH
spry4 WT chemical treatment: SU5402 10-13 somites somite ISH
spry4fh117/fh117 standard conditions 10-13 somites somite ISH
spry4fh117/fh117 standard conditions 10-13 somites somite ISH
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab-F59 IHC WT standard conditions Prim-5 migratory slow muscle precursor cell
IHC Prim-5 somite
IHC spry4fh117/fh117 standard conditions Prim-5 migratory slow muscle precursor cell
IHC Prim-5 somite
Ab1-eng IHC WT standard conditions Prim-5 muscle pioneer
IHC WT chemical treatment: SU5402 Prim-5 muscle pioneer
IHC spry4fh117/fh117 standard conditions Prim-5 muscle pioneer
IHC spry4fh117/fh117 chemical treatment: SU5402 Prim-5 muscle pioneer
Phenotype Details
Fish Conditions Stage Phenotype
WT chemical treatment: SU5402 Prim-5 muscle pioneer increased amount, abnormal
spry4fh117/fh117 standard conditions Prim-5 muscle pioneer decreased amount, abnormal
spry4fh117/fh117 chemical treatment: SU5402 Prim-5 muscle pioneer decreased amount, normal
Acknowledgments:
ZFIN wishes to thank the journal PLoS Genetics for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS Genet.