ZFIN ID: ZDB-FIG-121212-30
Lane et al., 2012 - Otx but not mitf transcription factors are required for zebrafish retinal pigment epithelium development. PLoS One   7(11):e49357 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
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Fish:
Knockdown Reagents:
Anatomical Terms:
Stage Range: 20-25 somites to Prim-5

Fig. 7 The combined knockdown of Otx and Mitf transcription factors creates a more severe phenotype.

(A) The effect of otx1a/otx2 knockdown was compared between wildtype, mitfaw2 and mitfbhu3857 single mutants, and mitfbhu3857;mitfaw2 double mutants. Morpholinos were injected at a total concentration of 2 ng/embryo using the same needle for all genotypes. Results from one representative experiment are shown. The percentage of severe RPE defects was significantly increased (P<0.0001) in the mitfbhu3857;mitfaw2 double mutant background compared to wildtype and single mitfa and mitfb mutants. Wildtype, N = 252; mitfa, N = 196; mitfb, N = 158; mitfa;mitfb, N = 170. (B,C) At 24 hpf dct expression is nearly eliminated in mitfbhu3857;mitfaw2 embryos injected with the otx1a/otx2 morpholino combination (C) compared to sibling embryos injected with control morpholino (B).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
dct mitfaw2/w2; mitfbhu3857/hu3857 standard conditions Prim-5 optic cup ISH
mitfaw2/w2; mitfbhu3857/hu3857 + MO1-otx2a + MO1-otx2b standard conditions Prim-5 optic cup ISH
otx2a WT standard conditions 20-25 somites central nervous system ISH
mitfaw2/w2 standard conditions 20-25 somites central nervous system ISH
mitfaw2/w2; mitfbhu3857/hu3857 standard conditions 20-25 somites central nervous system ISH
mitfbhu3857/hu3857 standard conditions 20-25 somites central nervous system ISH
otx2b WT standard conditions 20-25 somites central nervous system ISH
mitfaw2/w2 standard conditions 20-25 somites central nervous system ISH
mitfaw2/w2; mitfbhu3857/hu3857 standard conditions 20-25 somites central nervous system ISH
mitfbhu3857/hu3857 standard conditions 20-25 somites central nervous system ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS One