FIGURE

Fig. 2

ID

ZDB-FIG-121212-25

Publication

Lane et al., 2012 - Otx but not mitf transcription factors are required for zebrafish retinal pigment epithelium development

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Fig. 2

Otx morphant phenotypes.

(A–C) Eye phenotypes, assessed at 5 dpf, were scored as normal when the RPE displayed no visible defects (A), minor when coloboma was present but anterior and posterior ventral RPE were still contiguous (B), and major when the anterior and posterior ventral RPE were completely separated (C). (D) Histogram showing the eye defects induced by otx gene knockdown when injected at a morpholino concentration of 1.25 ng/embryo. Knockdown of individual otx genes caused only minor defects, while knockdown of otx1a and otx2 together, or all three otx genes together, produced a majority of embryos with major eye defects. otx1a, N = 108; otx1b, N = 230; otx2, N = 122; otx1a/otx1b, N = 222; otx1b/otx2, N = 256; otx1a/otx2, N = 214; otx1a/otx1b/otx2, N = 178.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-otx1 WT + MO1-otx2a WT + MO1-otx2b WT + MO1-otx1 + MO1-otx2a WT + MO1-otx1 + MO1-otx2b WT + MO1-otx2a + MO1-otx2b WT + MO1-otx1 + MO1-otx2a + MO1-otx2b
Knockdown Reagents:	MO1-otx1 MO1-otx2a MO1-otx2b
Observed In:	eye optic fissure retinal pigmented epithelium anterior side retinal pigmented epithelium posterior side retinal pigmented epithelium ventral region
Stage:	Day 5

Phenotype Detail

Acknowledgments

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