FIGURE

Fig. 6

ID

ZDB-FIG-121130-39

Publication

Lee et al., 2012 - Zebrafish larvae exposed to ginkgotoxin exhibit seizure-like behavior that is relieved by pyridoxal-5'-phosphate, GABA and anti-epileptic drugs

Other Figures

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Fig. 6

Ginkgotoxin-induced malformation of optic stalk and spinal cord neuron was partially reversed by PLP or GABA supplementation. Zebrafish embryos were raised in fish water (control) or water containing 500 μM ginkgotoxin added at 6 hpf (GT). (A,B) The development of embryos were characterized at 24 hpf using in-situ hybridization with pax2.1 probe (lateral view with anterior to the left). Development of ostic stalk and spinal cord neuron were interfered with in the ginkgotoxin-treated group (B) and the effect was reversed in the presence of 0.5 mM PLP (C) or 0.5 mM GABA (D). Adding PLP alone did not cause observable changes in embryos (E), whereas adding GABA alone to the water impeded the development of pax2.1-expressing tissues, especially optic stalk, otic vesicle and spinal cord neuron (F). (G,H) Confocal microscopy images of the nortochord in the trunk of Tg(alx:GFP) transgenic embryos at 3 dpf. OS, optic stalk; MHB, midbrain-hindbrain boundary; OV, otic vesicle; SPN, spinal cord neurons; PD, pronephric duct. Scale bar: 8 μm.

Expression Data

Gene:	pax2a
Fish:	AB
Conditions:	chemical treatment by environment: Ginkgotoxin chemical treatment by environment: gamma-aminobutyric acid chemical treatment by environment: pyridoxal 5'-phosphate
Anatomical Terms:	optic stalk spinal cord
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB nns1Tg
Conditions:	chemical treatment by environment: Ginkgotoxin chemical treatment by environment: gamma-aminobutyric acid chemical treatment by environment: pyridoxal 5'-phosphate
Observed In:	optic stalk spinal cord spinal cord interneuron
Stage Range:	Prim-5 to Protruding-mouth

Phenotype Detail

Acknowledgments

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