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ZFIN ID: ZDB-FIG-121101-15
Weiss et al., 2012 - Abnormal vasculature interferes with optic fissure closure in lmo2 mutant zebrafish embryos. Developmental Biology   369(2):191-198 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Knockdown Reagent:
Anatomical Term:
Stage: Long-pec
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Long-pec

Fig. 6 Ocular vessel dilation is flow dependent and interferes with optic fissure closure. (A–D) Single plane confocal images combined with transmitted light image of eyes of normal Tg(kdrl:EGFP) embryos (A and C) and lmo2vu270/vu270; Tg(kdrl:EGFP) embryos (B,D), uninjected (A and B) or injected with MO1-tnnt2a (C and D). Arrows point at GFP-positive endothelial cells (green) in close contact with optic fissure margins. (A′–D′) Images of the same embryos as in A–D. Arrows point at optic fissure margins. Embryos were treated with PTU to block pigmentation. All panels are lateral view, anterior to the left.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP lmo2vu270/vu270; s843Tg standard conditions Long-pec ocular blood vessel blood vessel endothelium IHC
lmo2vu270/vu270; s843Tg + MO1-tnnt2a standard conditions Long-pec ocular blood vessel blood vessel endothelium IHC
s843Tg standard conditions Long-pec ocular blood vessel blood vessel endothelium IHC
s843Tg + MO1-tnnt2a standard conditions Long-pec ocular blood vessel blood vessel endothelium IHC
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
lmo2vu270/vu270; s843Tg standard conditions Long-pec embryonic hemopoiesis decreased process quality, abnormal
Long-pec embryonic retina morphogenesis in camera-type eye decreased process quality, abnormal
Long-pec head edematous, abnormal
Long-pec head increased size, abnormal
Long-pec nucleate erythrocyte absent, abnormal
Long-pec ocular blood vessel increased diameter, abnormal
Long-pec optic fissure increased width, abnormal
Long-pec optic fissure malformed, abnormal
lmo2vu270/vu270; s843Tg + MO1-tnnt2a standard conditions Long-pec blood circulation arrested, abnormal
Long-pec embryonic hemopoiesis decreased process quality, abnormal
Long-pec head size, normal
Long-pec heart contraction arrested, abnormal
Long-pec nucleate erythrocyte absent, abnormal
Long-pec ocular blood vessel decreased diameter, abnormal
Long-pec optic fissure width, normal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 369(2), Weiss, O., Kaufman, R., Michaeli, N., and Inbal, A., Abnormal vasculature interferes with optic fissure closure in lmo2 mutant zebrafish embryos, 191-198, Copyright (2012) with permission from Elsevier. Full text @ Dev. Biol.