FIGURE

Fig. 4

ID

ZDB-FIG-121004-9

Publication

Jensen et al., 2012 - Opposing Effects of Circadian Clock Genes Bmal1 and Period2 in Regulation of VEGF-Dependent Angiogenesis in Developing Zebrafish

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Fig. 4

Involvement of the Notch Signaling Pathway in Circadian Clock-Regulated Vascular Sprouting(A) Zebrafish embryos received bmal1 (B1MO, 0.2 pmol/embryo), period2 (P2MO, 0.2 pmol/embryo) or vegf (VEGFMO, 0.1 pmol/embryo) morpholinos, either alone or in combination with DAPT (100 μM), rbpsuh morpholino (RbpsuhMO, 0.1 pmol/embryo), or in the mind-bomb background. The embryos were subsequently raised under the LD condition. Arrows point to ISV braches in each group. Scale bar is 100 μm.(B) Quantification of the average ± SEM. ISV density or number of branches/segment of 72 hpf embryos in (A) (average n = 23 embryos/group).(C) Zebrafish embryos received period2 (P2MO, 0.2 pmol/embryo) alone or in combination with DAPT (100 μM), rbpsuh morpholino (RbpsuhMO, 0.1 pmol/embryo), or in the mind-bomb background. The embryos were subsequently raised under LD or LL conditions. Arrows point to ISV braches in each group. Scale bar is 100 μm.(D) Quantification of the average ± SEM. ISV density or number of branches/segment of 72 hpf embryos in (C) (average n = 23 embryos/group). p < 0.05; p < 0.001; and p < 0.001. NS, not significant.

Expression Data

Gene:	EGFP
Fish:	y1Tg y1Tg + MO1-bmal1a y1Tg + MO1-per2 y1Tg + MO1-vegfaa mib1^ta52b/ta52b; y1Tg y1Tg + MO4-rbpja,rbpjb y1Tg + MO1-bmal1a + MO4-rbpja,rbpjb y1Tg + MO1-per2 + MO4-rbpja,rbpjb y1Tg + MO1-vegfaa + MO4-rbpja,rbpjb mib1^ta52b/ta52b; y1Tg + MO1-bmal1a mib1^ta52b/ta52b; y1Tg + MO1-per2 mib1^ta52b/ta52b; y1Tg + MO1-vegfaa (all 12)
Conditions:	chemical treatment: DAPT chemical treatment: DAPT, constant light constant light
Knockdown Reagents:	MO1-bmal1a MO1-per2 MO1-vegfaa MO4-rbpja,rbpjb
Anatomical Term:	intersegmental vessel
Stage:	Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	y1Tg mib1^ta52b/ta52b; y1Tg y1Tg + MO4-rbpja,rbpjb
Condition:	chemical treatment: DAPT
Knockdown Reagent:	MO4-rbpja,rbpjb
Observed In:	blood vessel intersegmental vessel vascular sprouts
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

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