FIGURE

Fig. 4

ID

ZDB-FIG-120831-4

Publication

Delaurier et al., 2012 - Histone deacetylase-4 is required during early cranial neural crest development for generation of the zebrafish palatal skeleton

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Fig. 4

pdgframRNA expression is down-regulated inhdac4MO-injected embryos, although the ligandpdgfaais unaffected. (A-D) mRNA in situ hybridizations, lateral views where anterior is towards the left, dorsal is upwards, Images are projections from confocal stacks. Dorsal and ventral margins and the eye margin of the embryo were outlined from brightfield images. (A) Uninjected embryos showed expression of pdgfra dorsal to the eye (d), posterior to the eye (p), and medial to the eye (m) at 15 hpf. (B) MO-injected embryos showed more limited expression of pdgfra, in particular loss of pdgfra expression medial to the eye (m) at 15 hpf (compare m in A with B). (C) At 17 hpf, uninjected embryos showed pdgfaa mRNA expression in the diencephalon (di), medial to the eye (m), and in the optic stalk anterior to the eye (os). (D) At 17 hpf, hdac4 MO-injected embryos had similar patterns of pdgfaa mRNA expression in the diencephalon (di), medial to the eye (m), and in the optic stalk (os). A and B, scale bar = 50 μm, C-D scale bar = 100 μm.

Expression Data

Genes:	pdgfaa pdgfra
Fish:	AB AB + MO1-hdac4 + MO2-hdac4
Knockdown Reagents:	MO1-hdac4 MO2-hdac4
Anatomical Terms:	diencephalon head dorsal region head medial region head posterior region head postero-medial region optic stalk
Stage Range:	10-13 somites to 14-19 somites

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ BMC Dev. Biol.