FIGURE

Fig. 5

ID

ZDB-FIG-120816-21

Publication

Lim et al., 2012 - Dorsal activity of maternal squint is mediated by a non-coding function of the RNA

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Fig. 5

Dorsal activity of sqt RNA requires Wnt/β-catenin but not Nodal signaling. (A-O) Expression of gsc in dome stage MZoep zebrafish embryos (A-E) shows that, compared with lacZ:glo RNA (A,F,K), injection of sqt^cz35:sqt (B,G,L), sqt^STOP:sqt (C,H,M), lacZ:sqt (D,I,N), or T-sqt:sqt (E,J,O) RNA expands the dorsal domain (B-E), similar to that in wild-type embryos (K-O). By contrast, ich embryos (F-J) show no/very sparse gsc expression for all injected RNAs. Arrowheads (A-E,K-O) mark the extent of gsc expression. A-O, animal pole views; A-E, K-O, dorsal to the right. (P) The angle of gsc in dome stage MZoep embryos after RNA injections. Each blue dot represents a single embryo. N indicates the number of injected embryos and μ(^o) shows mean gsc angles. (Q) Percentage embryos that manifest gsc expression at 30% epiboly after sqt RNA injections in control or ich mutant embryos. Scale bar: 100 μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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