Fig. S5
Mutant sqt RNA rescues early dorsal gene expression in sqt morphant embryos. Co-injection of sqtSTOP:sqt or lacZ:sqt RNA with sqtMO1 or sqtMO2 can rescue the loss of early gsc expression in sqt morphant embryos, in contrast to co-injection of lacZ:glo RNA with the sqt morpholinos. (A-D) Classification of gsc expression in injected embryos. Red arrowheads indicate the extent of gsc expression in the injected embryos. Animal pole views, dorsal to the right. (E) Histogram showing percentage embryos with dorsal gsc expression upon co-injection of sqt morpholinos with lacZ:glo, lacZ:sqt or sqtSTOP:sqt RNA. The number of embryos for each injection is shown at the top. Scale bar: 100 μm. |