FIGURE

Fig. 3

ID

ZDB-FIG-120815-42

Publication

Liu et al., 2012 - betaPix plays a dual role in cerebral vascular stability and angiogenesis, and interacts with integrin alphavbeta8

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Fig. 3

Loss of integrin α_v, integrin β₈ or combinatorial knockdown of both genes results in hemorrhage. As compared to uninjected controls in (A), 12 ng of itgav^e3i3 MO (B), or 4 ng of itgb8^e2i2 MO (C), leads to head hemorrhages at 50–52 hpf. (D–F) Suboptimal morpholino doses (3 ng of itgav^e3i3 MO (D) or 1 ng of itgb8^e2i2 (E)) results in small hemorrhages while double knockdown of both genes using these low doses results in numerous, larger hemorrhages (F, arrows). (G) Graphical representation of hemorrhage rate as a function of the dose of morpholino injected. Percentage of embryos with hemorrhages is shown above the bars. # represents the numbers of embryos scored for each analysis.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	TL + MO1-itgb8 TL + MO2-itgb8 TL + MO4-itgav TL + MO5-itgav TL + MO1-itgb8 + MO4-itgav
Knockdown Reagents:	MO1-itgb8 MO2-itgb8 MO4-itgav MO5-itgav
Observed In:	brain brain vasculature
Stage:	Long-pec

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 363(1), Liu, J., Zeng, L., Kennedy, R.M., Gruenig, N.M., and Childs, S.J., betaPix plays a dual role in cerebral vascular stability and angiogenesis, and interacts with integrin alphavbeta8, 95-105, Copyright (2012) with permission from Elsevier. Full text @ Dev. Biol.