Fig. S1
Expression of bmp2a, bmp2b and ihha is unchanged in the gut of miR145 morphants Lateral view of whole mount in situ hybridization shows that bmp2a, bmp2b and ihha remain similar expression level after miR145 is knocked down in the gut of 96 hpf embryos. Sections to the right of the wholemount embryo represent longitudinal sections of the whole mount embryos. Sections show the expression of bmp2a and bmp2b is only restricted to smooth muscle cell (SMC; indicates by arrowhead) and ihha is expressed in the epithelial cell (EC; indicated by arrow). The magnification for wholemount embryos and sections is shown by the scale bar in K and L respectively. |
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Stage: | Day 4 |
Reprinted from Developmental Biology, 367(2), Zeng, L., and Childs, S.J., The smooth muscle microRNA miR-145 regulates gut epithelial development via a paracrine mechanism, 178-186, Copyright (2012) with permission from Elsevier. Full text @ Dev. Biol.