header logo image header logo text
Downloads Login
Research
General Information
ZIRC
ZFIN ID: ZDB-FIG-120329-36
Zhu et al., 2012 - Activated ALK Collaborates with MYCN in Neuroblastoma Pathogenesis. Cancer Cell   21(3):362-373 Full text @ Cancer Cell
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage: Protruding-mouth
PHENOTYPE:
Fish:
Observed In:
Stage: Protruding-mouth

Fig. 4

MYCN Expression Causes Sympathoadrenal Cell Loss (A) DβH transgenic line. Oblique views of dβh RNA expression (left panels); lateral views of EGFP expression in merged confocal-brightfield images (middle panels); dorsal views of th RNA expression (right panels). Arrows point to the SCG, and arrowheads point to the CG. Scale bar represents 100 μm. (B) MYCN transgenic line. MYCN expression causes loss of cells in the SCG (arrows). Scale bar represents 100 μm. (C) ALK transgenic line. ALK expression does not interfere with the SCG development (arrows). Scale bar represents 100 μm. (D) MYCN;ALK transgenic line. Loss of cells in the SCG is not rescued by activated ALK expression (arrows). Scale bar represents 100 μm. AAC, arch-associated catecholaminergic neurons; CG (arrowheads), cranial ganglia; DA, diencephalic dopaminergic neurons; e, ear; LC, locus coeruleus; MO, medulla oblongata; r, retina; SCG, superior cervical ganglion. See also Figure S4 and Table S1.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
dbh zdf15Tg(AB) standard conditions Protruding-mouth locus coeruleus ISH
Protruding-mouth medulla oblongata ISH
Protruding-mouth retina ISH
Protruding-mouth superior cervical ganglion ISH
zdf16Tg(AB) standard conditions Protruding-mouth locus coeruleus ISH
Protruding-mouth medulla oblongata ISH
Protruding-mouth retina ISH
zdf16Tg; zdf17Tg(AB) standard conditions Protruding-mouth locus coeruleus ISH
Protruding-mouth medulla oblongata ISH
Protruding-mouth retina ISH
zdf17Tg(AB) standard conditions Protruding-mouth locus coeruleus ISH
Protruding-mouth medulla oblongata ISH
Protruding-mouth retina ISH
Protruding-mouth superior cervical ganglion ISH
EGFP zdf15Tg(AB) standard conditions Protruding-mouth cranial ganglion IFL
Protruding-mouth locus coeruleus IFL
Protruding-mouth medulla oblongata IFL
Protruding-mouth superior cervical ganglion IFL
zdf16Tg(AB) standard conditions Protruding-mouth cranial ganglion IFL
Protruding-mouth locus coeruleus IFL
Protruding-mouth medulla oblongata IFL
zdf16Tg; zdf17Tg(AB) standard conditions Protruding-mouth cranial ganglion IFL
Protruding-mouth medulla oblongata IFL
Protruding-mouth Not Detected superior cervical ganglion IFL
zdf17Tg(AB) standard conditions Protruding-mouth cranial ganglion IFL
Protruding-mouth locus coeruleus IFL
Protruding-mouth medulla oblongata IFL
Protruding-mouth superior cervical ganglion IFL
th zdf15Tg(AB) standard conditions Protruding-mouth diencephalon dopaminergic neuron ISH
Protruding-mouth retina ISH
Protruding-mouth superior cervical ganglion ISH
zdf16Tg(AB) standard conditions Protruding-mouth diencephalon dopaminergic neuron ISH
Protruding-mouth retina ISH
Protruding-mouth superior cervical ganglion ISH
zdf16Tg; zdf17Tg(AB) standard conditions Protruding-mouth diencephalon dopaminergic neuron ISH
Protruding-mouth retina ISH
Protruding-mouth Not Detected superior cervical ganglion ISH
zdf17Tg(AB) standard conditions Protruding-mouth diencephalon dopaminergic neuron ISH
Protruding-mouth retina ISH
Protruding-mouth superior cervical ganglion ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
zdf16Tg(AB) standard conditions Protruding-mouth superior cervical ganglion absent, abnormal
zdf16Tg; zdf17Tg(AB) standard conditions Protruding-mouth superior cervical ganglion absent, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Cancer Cell for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Cancer Cell, 21(3), Zhu, S., Lee, J.S., Guo, F., Shin, J., Perez-Atayde, A.R., Kutok, J.L., Rodig, S.J., Neuberg, D.S., Helman, D., Feng, H., Stewart, R.A., Wang, W., George, R.E., Kanki, J.P., and Look, A.T., Activated ALK Collaborates with MYCN in Neuroblastoma Pathogenesis, 362-373, Copyright (2012) with permission from Elsevier. Full text @ Cancer Cell