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ZFIN ID: ZDB-FIG-120216-56
Langenbacher et al., 2012 - Sodium pump activity in the yolk syncytial layer regulates zebrafish heart tube morphogenesis. Developmental Biology   362(2):263-270 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Anatomical Terms:
Stage: 20-25 somites
PHENOTYPE:
Fish:
Observed In:
Stage: 20-25 somites

Fig. 3

Sodium pump activity is required for extracellular matrix deposition around the cardiac cone. (A,B) Transverse microtome sections of 20S stage wild type and atp1a1m883 embryos stained with methylene blue. In wild type embryos, sulfated proteoglycans are stained pink by methylene blue, allowing visualization of the abundant extracellular matrix present around the cardiomyocytes of the cardiac cone (*) at the 20S stage (A). In atp1a1m883 mutants, the cardiac cone forms normally (*), but thickened extracellular matrix is completely absent (B). (C,D) Transverse vibratome sections of 20S stage wild type and atp1a1m883 Tg(myl7:EGFP) embryos. Wild type embryos (C) have Fibronectin protein (green) surrounding the cardiomyocytes of the cardiac cone (blue), while atp1a1m883 mutants (D) have extremely reduced Fibronectin protein deposition. (E,F) Dorsal views of fn1 expression in 20S stage wild type and atp1a1m883 embryos. Fn1 is expressed in the cardiac region of wild type embryos (E), but is dramatically reduced in atp1a1m883 mutants (F). (G,H) Lateral views of fn1 expression in 20S stage wild type and atp1a1m883 embryos. Both wild type (G) and atp1a1m883 mutant (H) embryos exhibit fn1 expression in posterior structures including the yolk extension and tail.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
fn1a WT standard conditions 20-25 somites extension ISH
20-25 somites heart rudiment ISH
20-25 somites tail bud ISH
atp1a1a.1m883/m883 standard conditions 20-25 somites extension ISH
20-25 somites heart rudiment ISH
20-25 somites tail bud ISH
atp1a1a.1m883/m883; twu34Tg standard conditions 20-25 somites heart primordium IHC
twu34Tg standard conditions 20-25 somites heart primordium IHC
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
atp1a1a.1m883/m883 standard conditions 20-25 somites extracellular matrix organization disrupted, abnormal
20-25 somites heart rudiment lacks parts or has fewer parts of type heart rudiment extracellular matrix, abnormal
20-25 somites heart rudiment extracellular matrix composition, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 362(2), Langenbacher, A.D., Huang, J., Chen, Y., and Chen, J.N., Sodium pump activity in the yolk syncytial layer regulates zebrafish heart tube morphogenesis, 263-270, Copyright (2012) with permission from Elsevier. Full text @ Dev. Biol.