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ZFIN ID: ZDB-FIG-120203-1
Weiner et al., 2012 - Fishing the molecular bases of Treacher Collins syndrome. PLoS One   7(1):e29574 Full text @ PLoS One
Anatomical Terms:
Stage Range: 2-cell to Day 5

Fig. 2

Developmental expression pattern of tcof1 by whole mount in situ hybridization.

Lateral (AI, K, L, NQ, S) and dorsal (J, M, R and T) views (anterior regions to the left) of zebrafish embryos hybridized with anti-sense (AD, I, J, L, M, O, P, and R–T) or sense (EH, K, N and Q) zebrafish tcof1 probes. The following stages were analyzed: 2-cell stage (A and E), 6 hpf (B and F), 10 hpf (C and G), 13 hpf (D and H), 24 hpf (IK), 48 hpf (LN), 72 hpf (OR), and 120 hpf (ST). Abbreviations: cs, craniofacial structures; de, diencephalic region; e, eye; g, gut; mb, midbrain; hb, hindbrain; l, lenses; op, olfactory pits; ot, optic tectum; pa, pharyngeal arches; pf, pectoral fin buds. Scale bar: 180 μm for AH; 210 μm for I, J and P; 266 μm for K; 320 μm for L, M and R; 380 μm for N, O, and ST; 530 μm for Q.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
nolc1 WT standard conditions 2-cell whole organism ISH
Shield whole organism ISH
Bud whole organism ISH
10-13 somites diencephalon ISH
10-13 somites immature eye ISH
10-13 somites whole organism ISH
Prim-5 eye ISH
Prim-5 hindbrain ISH
Prim-5 midbrain ISH
Prim-5 pharyngeal arch ISH
Long-pec cranium ISH
Long-pec olfactory pit ISH
Long-pec optic tectum ISH
Long-pec pectoral fin bud ISH
Long-pec pharyngeal arch ISH
Long-pec retina ISH
Protruding-mouth cranium ISH
Protruding-mouth lens ISH
Protruding-mouth optic tectum ISH
Day 5 cranium ISH
Day 5 gut ISH
Day 5 lens ISH
Antibody Labeling Details No data available
Phenotype Details No data available
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One