FIGURE

Fig. S2

ID

ZDB-FIG-120126-62

Publication

Lindeman et al., 2011 - Prepatterning of Developmental Gene Expression by Modified Histones before Zygotic Genome Activation

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Fig. S2

Additional validation of H3K4me3 and H3K27me3 genomic association at the pre-MBT stage (related to Figures 1 and 2). (A) Enrichment in histone H3 trimethylation and RNAPII on the hoxb locus (boxed) at pre-MBT, MBT and post-MBT stages (MA2C scores). Red bars indicate out-of-scale MA2C scores. Primary transcripts are shown with TSS and transcription orientation (arrow). (B) ChIP-qPCR analysis of H3K4me3 (Millipore antibody) and H3K27me3 (Diagenode antibody) enrichment (mean from triplicate ChIPs). (C) Immunofluorescence detection of H3K4me3 in the animal pole of embryos at indicated stages (Cell Signaling antibody). Arrows point to examples of labeled nuclei. Scale bar, 10 μm.

Expression Data

Genes:	fmr1 hoxa9b hoxa11b myh6 shha shhb
Fish:	WT
Anatomical Term:	whole organism
Stage:	256-cell

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Cell, 21(6), Lindeman, L.C., Andersen, I.S., Reiner, A.H., Li, N., Aanes, H., Ostrup, O., Winata, C., Mathavan, S., Muller, F., Aleström, P., and Collas, P., Prepatterning of Developmental Gene Expression by Modified Histones before Zygotic Genome Activation, 993-1004, Copyright (2011) with permission from Elsevier. Full text @ Dev. Cell