ZFIN ID: ZDB-FIG-111215-9
Oehlers et al., 2011 - The inflammatory bowel disease (IBD) susceptibility genes NOD1 and NOD2 have conserved anti-bacterial roles in zebrafish. Disease models & mechanisms   4(6):832-41 Full text @ Dis. Model. Mech.
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Condition:
Knockdown Reagents:
Observed In:
Stage Range: Protruding-mouth to Day 6

Fig. 2 Nod1 and Nod2 are required for systemic innate immunity in zebrafish embryos. (A) Time course of Tg(lyzC:dsRed)50 embryos injected with PBS or 200 CFU of GFP-tagged S. enterica into the yolk sac at 2 dpf. Scale bar: 1 mm. (B) Survival of embryos infected by yolk sac injection. Data represent means of triplicate replicates; 30 embryos per treatment group. All error bars represent a 95% confidence interval. (C) Scatter plots showing S. enterica CFU counts recovered from individual embryos infected by yolk sac injection with 200 CFU of GFP-tagged S. enterica at 2 dpf. Data represent three biological replicates. Error bars represent s.e.m.; statistically significant differences of P<0.05 (*), P<0.01 (**) and P<0.001 (***) as determined by ANOVA are marked.

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
nz50Tg bacterial treatment by injection: Salmonella enterica subsp. enterica serovar Typhimurium Protruding-mouth - Day 6 whole organism viability, abnormal
nz50Tg + MO1-nod1 bacterial treatment by injection: Salmonella enterica subsp. enterica serovar Typhimurium Protruding-mouth - Day 6 defense response to bacterium disrupted, abnormal
Protruding-mouth - Day 6 neutrophil mediated immunity disrupted, abnormal
Protruding-mouth - Day 6 whole organism viability, abnormal
nz50Tg + MO1-nod2 bacterial treatment by injection: Salmonella enterica subsp. enterica serovar Typhimurium Protruding-mouth - Day 6 defense response to bacterium disrupted, abnormal
Protruding-mouth - Day 6 neutrophil mediated immunity disrupted, abnormal
Protruding-mouth - Day 6 whole organism viability, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Disease models & mechanisms for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Dis. Model. Mech.