ZFIN ID: ZDB-FIG-111215-11
Oehlers et al., 2011 - The inflammatory bowel disease (IBD) susceptibility genes NOD1 and NOD2 have conserved anti-bacterial roles in zebrafish. Disease models & mechanisms   4(6):832-41 Full text @ Dis. Model. Mech.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage: Day 4
PHENOTYPE:
Fish:
Condition:
Knockdown Reagents:
Observed In:
Stage: Day 5

Fig. 4 Depletion of zebrafish Nod1 or Nod2 results in reduced expression of duox and increased S. enterica burden following infection in larvae. (A) Expression of duox examined by qPCR at 6 and 24 hpi. Data represents at least triplicate pools of 10 to 20 larvae. Error bars represent s.e.m.; statistically significant differences of P<0.05 (*), P<0.01 (**) and P<0.0001 (***) as determined by ANOVA are marked. (B) Expression of duox in 4-dpf control and morphant larvae examined by in situ hybridization. Green arrows indicate thyroid expression; red arrows indicate intestinal epithelial cell expression. (C) Whole-larvae and intracellular bacterial recovery from 6 and 24 hpi larvae. Triplicate groups of five larvae were analyzed for each biological replicate represented by a single dot. Error bars represent s.e.m.; statistically significant differences of P<0.05 (*) and P<0.01 (**) as determined by ANOVA are marked.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
duox WT control Day 4 epidermis ISH
Day 4 intestinal epithelium ISH
Day 4 thyroid follicle ISH
WT standard conditions Day 4 epidermis ISH
Day 4 intestinal epithelium ISH
Day 4 thyroid follicle ISH
WT + MO1-nod1 standard conditions Day 4 epidermis ISH
Day 4 intestinal epithelium ISH
Day 4 thyroid follicle ISH
WT + MO1-nod2 standard conditions Day 4 epidermis ISH
Day 4 intestinal epithelium ISH
Day 4 thyroid follicle ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-nod1 bacterial treatment by exposure to environment: Salmonella enterica subsp. enterica serovar Typhimurium Day 5 defense response to bacterium disrupted, abnormal
WT + MO1-nod2 bacterial treatment by exposure to environment: Salmonella enterica subsp. enterica serovar Typhimurium Day 5 defense response to bacterium disrupted, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Disease models & mechanisms for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Dis. Model. Mech.