Fig. 2 Nod1 and Nod2 are required for systemic innate immunity in zebrafish embryos. (A) Time course of Tg(lyzC:dsRed)50 embryos injected with PBS or 200 CFU of GFP-tagged S. enterica into the yolk sac at 2 dpf. Scale bar: 1 mm. (B) Survival of embryos infected by yolk sac injection. Data represent means of triplicate replicates; 30 embryos per treatment group. All error bars represent a 95% confidence interval. (C) Scatter plots showing S. enterica CFU counts recovered from individual embryos infected by yolk sac injection with 200 CFU of GFP-tagged S. enterica at 2 dpf. Data represent three biological replicates. Error bars represent s.e.m.; statistically significant differences of P<0.05 (*), P<0.01 (**) and P<0.001 (***) as determined by ANOVA are marked.
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